Effects of Polycaprolactone-Tricalcium Phosphate, Recombinant Human Bone Morphogenetic Protein-2 and Dog Mesenchymal Stem Cells on Bone Formation: Pilot Study in Dogs.
10.3349/ymj.2009.50.6.825
- Author:
Sun Jong KIM
1
;
Myung Rae KIM
;
Jin Sub OH
;
Inho HAN
;
Sang Wan SHIN
Author Information
1. Department of Oral and Maxillofacial Surgery, Ewha Womans University School of Medicine, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
PCL-TCP scaffold;
dog MSCs;
recombinant human bone morphogenic protein-2;
auto-fibrin glue;
bone formation
- MeSH:
Animals;
Bone Morphogenetic Proteins/*pharmacology;
Calcium Phosphates/*pharmacology;
Cell Proliferation/drug effects;
Cell Survival/drug effects;
Cells, Cultured;
Dogs;
Fibrin Tissue Adhesive/pharmacology;
Humans;
Mesenchymal Stem Cell Transplantation;
Mesenchymal Stem Cells/*cytology/*drug effects/physiology;
Microscopy, Fluorescence;
Osteogenesis/*drug effects;
Polyesters/*pharmacology;
Recombinant Proteins/*pharmacology;
Transforming Growth Factor beta/*pharmacology
- From:Yonsei Medical Journal
2009;50(6):825-831
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: The aim of this study was to evaluate the survival, proliferation, and bone formation of dog mesenchymal stem cells (dMSCs) in the graft material by using Polycaprolactone-tricalcium phosphate (PCL-TCP), auto-fibrin glue (AFG), recombinant human bone morphogenetic protein-2 (rhBMP-2), and dMSCs after a transplantation to the scapula of adult beagle dogs. MATERIALS AND METHODS: The subjects were two beagle dogs. Total dose of rhBMP-2 on each block was 10 microg with 50 microg/mg concentration. The cortical bone of the scapula of the dog was removed which was the same size of PCL-TCP block (Osteopore International Pte, Singapore; 5.0x5.0x8.0 mm in size), and the following graft material then was fixed with orthodontic mini-implant, Dual-top(R) (Titanium alloy, Jeil Co. Seoul, Korea). Four experimental groups were prepared for this study, Group 1: PCL-TCP + aFG; Group 2: PCL-TCP + aFG + dMSCs; Group 3: PCL-TCP + aFG + dMSCs + rhBMP-2; Group 4: PCL-TCP + aFG + dMSCs + rhBMP-2 + PCL membrane. The survival or proliferation of dMSCs cells was identified with an extracted tissue through a fluorescence microscope, H-E staining and Von-Kossa staining in two weeks and four weeks after the transplantation. RESULTS: The survival and proliferation of dMSCs were identified through a fluorescence microscope from both Group 1 and Group 2 in two weeks and four weeks after the transplantation. Histological observation also found that the injected cells were proliferating well in the G2, G3, and G4 scaffolds. CONCLUSION: This study concluded that bone ingrowth occurred in PCL-TCP scaffold which was transplanted with rhBMP-2, and MSCs did not affect bone growth. More sufficient healing time would be needed to recognize effects of dMSCs on bone formation.
