The Protective Effect of Sodium Hyaluronate on the Cartilage of Rabbit Osteoarthritis by Inhibiting Peroxisome Proliferator-Activated Receptor-Gamma Messenger RNA Expression.
10.3349/ymj.2009.50.6.832
- Author:
Jian Lin ZHOU
1
;
Shi Qing LIU
;
Bo QIU
;
Qiong Jie HU
;
Jiang Hua MING
;
Hao PENG
Author Information
1. Department of Orthopedics, Renmin Hospital of Wuhan University, Wuhan, Hubei, China. zhoujianlin1688@yahoo.com.cn
- Publication Type:Original Article
- Keywords:
Sodium hyaluronate;
Peroxisome proliferators-activated receptor gamma;
cartilage;
osteoarthritis
- MeSH:
Animals;
Cartilage/*drug effects/*metabolism;
Gene Expression/drug effects/genetics;
Hyaluronic Acid/*pharmacology/therapeutic use;
Microscopy;
Osteoarthritis/*drug therapy/metabolism;
PPAR gamma/*genetics;
RNA, Messenger/*genetics;
Rabbits;
Random Allocation;
Reverse Transcriptase Polymerase Chain Reaction;
Viscosupplements/*pharmacology/therapeutic use
- From:Yonsei Medical Journal
2009;50(6):832-837
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: The purpose of this study was to study the protective effect and influence of sodium hyaluronate (Na-HA) on mRNA expression of peroxisome proliferators-activated receptor gamma (PPAR-gamma) in cartilage of rabbit osteoarthritis (OA) model. MATERIALS AND METHODS: Forty eight white rabbits were randomly divided into A, B, and C groups. Group A was normal control group, B and C groups underwent unilateral anterior cruciate ligament transection (ACLT). The rabbits in group B were injected normal saline after ACLT; and Group C received intraarticular1% sodium hyaluronate (HA) injection 5 weeks after surgery, 0.3 mL once a week. At 11th week after surgery, all the rabbits were sacrificed. The cartilage changes on the medial femoral condyles were graded separately. Cartilage sections were stained with safranin-O and HE, and messenger RNA (mRNA) expression of PPAR-gamma was detected by using real time polymerase chain reaction (Real Time-PCR). RESULTS: Cartilage degeneration in group B was significantly more severe than in A and C injection group. The grey value of Safranin-O of B group was higher than A and C groups. Expression of PPAR-gamma mRNA in group B was higher than group A and C. CONCLUSION: This study shows that Na-HA has a protective effect on articular cartilage degeneration, and the inhibitory effect on the PPAR-gamma mRNA expression may be one of therapeutic mechanism of Na-HA.
