Increased Prooxidative Activity Stimulating the Oxidative Modification of Proteins in the Umbilical Venous Plasma and Placenta of Preeclampsia.
- Author:
Eun Mi KIM
1
;
Yoon Ha KIM
;
Bong Whan AHN
;
Sung Yeul YANG
;
Cheol Hong KIM
;
Moon Kyoung CHO
;
Seok Mo KIM
;
Tae Bok SONG
Author Information
1. Department of Obstetrics and Gynecology, Chonnam National University Medical School, Gwangju, Korea. kimyh@chonnam.ac.kr
- Publication Type:Original Article
- Keywords:
Preeclampsia;
Umbilical venous plasma;
Placenta;
Lipid peroxide;
Oxidative modification of protein
- MeSH:
Cephalosporins;
Cephalothin;
Female;
Humans;
Moxalactam;
Placenta*;
Plasma*;
Pre-Eclampsia*;
Pregnancy;
Protein Carbonylation
- From:Korean Journal of Perinatology
2004;15(1):27-33
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: This study was performed to compare the prooxidative activity stimulating the protein carbonyl formation by cephalosporins in the umbilical venous and placenta of preeclampsia with that of normal pregnancy. METHODS: Lipid peroxide levels in the umbilical venous plasma and placental tissue homogenates of normal pregnancy (n=12) and preeclampsia (n=12) were measured by thiobarbituric acid reaction. The basal protein carbonyl contents in the umbilical venous plasma and placental tissue homogenates of normal pregnancy (n=12) and preeclampsia (n=12) were determined by the 2,4-dinitrophenylhydrazine (DNPH) method. After samples of them were mixed and incubated up to 5 hours with 0.2 mL of 1 mM moxalactam or cephalothin, the protein carbonyl contents in them were measured by DNPH. RESULTS: Protein carbonyls formation by moxalactam and cephalothin in the umbilical venous plasma and of women with preeclampsia were significantly higher than that of women with normal pregnancy (8.5+/-2.0 vs. 6.6+/-1.4 nmol/mg protein, p<0.05, 7.6+/-1.6 vs. 6.2+/-1.2 nmol/mg protein, p<0.05). Protein carbonyls formation by moxalactam and cephalothin in the placental tissue homogenates of women with preeclampsia were significantly higher than that of women with normal pregnancy (17.6+/-5.3 vs. 13.0+/-4.2 nmol/mg protein, p<0.05, 16.1+/-5.2 vs. 12.5+/-4.4 nmol/mg protein, p<0.05). There were significant positive correlations between lipid peroxide and cephalosporins induced protein carbonyls levels of umbilical venous plasma, and placental tissue homogenates (p<0.01). CONCLUSION: These results suggest that increase in the prooxidative activity stimulating the oxidative modification of proteins in placenta may be involved in the pathogenesis of preecalmpsia.
