Regulation of betaig-h3 Production in Rheumatoid Synovitis by Inflammatory Mediators.
- Author:
Young Mo KANG
1
;
Sung Il KIM
;
Jeong Seup KIM
;
Dong Wan YOU
;
Kheum Hee SA
;
Eun Ju PARK
;
Sung Uk KIM
;
Jae Seok SEO
;
Seung Woo HAN
;
Eon Jeong NAM
;
Hee Soo KYUNG
;
Moon Gyu KIM
;
In San KIM
;
Jung Chol KIM
Author Information
1. Department of Internal Medicine, Kyungpook National University School of Medicine, Daegu, Korea. ymkang@knu.ac.kr
- Publication Type:Original Article
- Keywords:
Rheumatoid arthritis;
Transforming growth factor-beta-inducible gene-h3;
Fibroblast-like synoviocyte
- MeSH:
Arthritis, Rheumatoid;
Cytokines;
Dexamethasone;
Enzyme-Linked Immunosorbent Assay;
Flow Cytometry;
Fluorescent Antibody Technique;
Humans;
Interleukin-10;
Interleukin-4;
Interleukin-6;
Joints;
Osteoarthritis;
Synovial Membrane;
Synovitis*;
Transforming Growth Factor beta;
Transforming Growth Factor beta1;
Tumor Necrosis Factor-alpha
- From:The Journal of the Korean Rheumatism Association
2005;12(2):73-82
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: To investigate the expression pattern of transforming growth factor-beta-inducible gene-h3 (betaig-h3) within rheumatoid synovial tissue and the regulation of betaig-h3 synthesis in fibroblast-like synoviocyte (FLS). METHODS: Synovial tissues obtained from patients with rheumatoid arthritis and osteoarthritis were obtained during joint replacement surgery. betaig-h3 expression was evaluated with immunohistochemical stain. FLS was isolated from synovial tissues and stimulated with cytokines including TGF-beta, TNF-alpha, IL-1beta, IFN-gamma, IL-6, IL-4, and IL-10. betaig-h3 synthesis was measured using semiquantitative RT-PCR, ELISA, immunofluorescence stain, and flow cytometry. RESULTS: Expression of betaig-h3 was diffuse and abundant in both lining and sublining layers of rheumatoid synovium, which was more prominent than those of osteoarthritis. Production of betaig-h3 in FLS was regulated by TGF-beta1 in a dose-dependent manner and was highest at 5 ng/mL of TGF-beta1. TNF-alpha and IL-1beta upregulated the production of betaig-h3 from FLS synergistically with TGF-beta1 but other cytokines such as IL-4, IL-6, IL-10 did not affect. betaig-h3 synthesis was efficiently inhibited by dexamethasone at higher dose (100 nM) but not by cyclosporine-A. CONCLUSION: Production of betaig-h3, which is highly upregulated in rheumatoid synovitis, is differentially regulated by inflammatory cytokines.
