IgE Binding Reactivity of Peptide Fragments of Bla g 4, a Major German Cockroach Allergen.
- Author:
Kwang Hyun SHIN
1
;
Kyoung Yong JEONG
;
Chein Soo HONG
;
Tai Soon YONG
Author Information
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords: Blattella germanica; Allergen; B-cell epitope; IgE; lipocalin
- MeSH: Adolescent; Adult; Allergens/chemistry/genetics/*immunology; Amino Acid Sequence; Animals; Child; Cockroaches/*immunology; *Epitope Mapping; Escherichia coli/genetics/metabolism; Female; Humans; Hypersensitivity/*immunology; Immunoglobulin E/*immunology; Insect Proteins/chemistry/genetics/*immunology; Male; Middle Aged; Molecular Sequence Data; Sequence Alignment; Young Adult
- From:The Korean Journal of Parasitology 2009;47(1):31-36
- CountryRepublic of Korea
- Language:English
- Abstract: Cockroaches have been recognized as a major cause of asthma. Bla g 4 is one of the most important German cockroach allergens. The aim of this study is to investigate IgE reactivity to the recombinant Bla g 4 (rBla g 4) in the sera of allergic patients and identify linear IgE binding epitope. For protein expression, full-length Bla g 4 (EF202172) was divided into 5 overlapping peptide fragments (E1: aa 1-100, E2: aa 34-77, E3: aa 74-117, E4: aa 114-156, and E5: aa 153-182). The full-length and 5 peptide fragments of Bla g 4 was generated by PCR and over-expressed in E. coli BL21 (DE3). The IgE binding reactivities of the full-length and peptide fragments were measured by ELISA using 32 serum samples of cockroach allergy. The sera of 8 patients (25%) reacted with rBla g 4. Four sera (100%) showed IgE-binding reactivity to full-length and peptide fragment 4, and 2 sera (50%) reacted with peptide fragment 2. One (20%) serum reacted with peptide fragment 3. The results of ELISA using overlapping recombinant fragments indicated that the epitope region was located at amino acid sequences 34-73 and 78-113, and major IgE epitope of Bla g 4 was located at amino acid sequences 118-152 of C-terminal. B-cell epitope analysis of German cockroach allergen Bla g 4 could contribute to the strategic development of more specific and potentially efficacious immunotherapy.
