siMacro: A Fast and Easy Data Processing Tool for Cell-Based Genomewide siRNA Screens.
- Author:
Nitin Kumar SINGH
1
;
Bo Yeun SEO
;
Mathukumalli VIDYASAGAR
;
Michael A WHITE
;
Hyun Seok KIM
Author Information
- Publication Type:Original Article
- Keywords: high-throughput screening assays; RNA interference
- MeSH: High-Throughput Screening Assays; RNA Interference; RNA, Small Interfering
- From:Genomics & Informatics 2013;11(1):55-57
- CountryRepublic of Korea
- Language:English
- Abstract: Growing numbers of studies employ cell line-based systematic short interfering RNA (siRNA) screens to study gene functions and to identify drug targets. As multiple sources of variations that are unique to siRNA screens exist, there is a growing demand for a computational tool that generates normalized values and standardized scores. However, only a few tools have been available so far with limited usability. Here, we present siMacro, a fast and easy-to-use Microsoft Office Excel-based tool with a graphic user interface, designed to process single-condition or two-condition synthetic screen datasets. siMacro normalizes position and batch effects, censors outlier samples, and calculates Z-scores and robust Z-scores, with a spreadsheet output of >120,000 samples in under 1 minute.
