Development of a Gene Therapy Method for Cervical Cancer Using Attenuated Coxsackievirus B3 as a Vector System.
10.4167/jbv.2011.41.2.123
- Author:
Seung Hyun SHIM
1
;
Yeon Jung KIM
;
Dae Sun KIM
;
Jae Hwan NAM
Author Information
1. Department of Biotechnology, The Catholic University, Gyeonggi-do, Korea. jhnam@catholic.ac.kr
- Publication Type:Original Article
- Keywords:
Attenuated coxsackievirus B3;
YYFF;
Gene therapy;
Vector system;
Cervical cancer
- MeSH:
Animals;
Cell Culture Techniques;
Gene Expression;
Genes, vif;
Genetic Therapy;
Human papillomavirus 16;
Mice;
Mice, Nude;
Uterine Cervical Neoplasms
- From:Journal of Bacteriology and Virology
2011;41(2):123-130
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
We previously reported the development of an attenuated coxsackievirus B3, known as YYFF, which functioned as a viral vector system for foreign gene expression. In this study, we demonstrated the potential use of YYFF as a gene therapy vector. Recombinant YYFF was constructed to express the human papillomavirus 16 (HPV16) E7 gene, referred to as YYFF-HPV16-E7. Growth of YYFF-HPV16-E7 resembled the wild type, YYFF, and it expressed HPV16-E7 in cell culture. When YYFF-HPV16-E7 was directly injected into TC-1-transplanted C57/BL6 mice, there was no reduction in tumor size, because of the non-growth of YYFF in C57/BL6 mice. However, when YYFF-HPV16-E7-induced immune cells/serum that originated from BALB/c mice was passively delivered into BALB/c background TC-1-transplanted nude mice, it reduced the size of cervical tumors in the nude mice. This study indicates the potential use of YYFF-HPV16-E7 as a gene therapy agent for treating HPV-induced cervical cancer.
