Evaluation of the safety of non-thermal atmospheric-pressure plasma in hairless mouse tissues.
10.11149/jkaoh.2014.38.3.148
- Author:
Sang Rye PARK
1
;
Gyoo Cheon KIM
;
Byul Bora CHOI
;
Ji Young KIM
Author Information
1. Department of Dental Hygiene, Kyungnam College of Information & Technology, Busan, Korea. kjy1@kit.ac.kr
- Publication Type:Original Article
- Keywords:
Candida albicans;
Hematoxylin and eosin stain;
Masson's trichrome stain;
Non-thermal atmospheric pressure plasma;
Scanning electron microscopy
- MeSH:
Animals;
Biopsy;
Candida albicans;
Collagen;
Coloring Agents;
Dentistry;
Eosine Yellowish-(YS);
Hematoxylin;
Inflammation;
Membranes;
Mice;
Mice, Hairless*;
Microscopy, Electron, Scanning;
Optical Fibers;
Plasma Gases;
Plasma*;
Skin;
Thermometers
- From:Journal of Korean Academy of Oral Health
2014;38(3):148-153
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVES: The aim of the present study was to evaluate the stability of non-thermal atmospheric-pressure plasma on Candida albicans in hairless mouse-2 (HRM-2) tissues. METHODS: HRM-2 mice were subjected to non-thermal atmospheric-pressure plasma jet treatment using an optical fiber probe and monitored using a thermometer. The skin of HRM-2 mice was treated with plasma jet for 0, 60, 180, and 300 s per day for 5 days. After plasma treatment, morphological changes in Candida albicans on the skin of these mice were examined using a scanning electron microscope. Biopsy of the plasma-treated skin was performed and the tissues were histologically analyzed using hematoxylin and eosin (H&E) and Masson's trichrome stains. RESULTS: The scanning electron microscopic images revealed the morphological changes in the membrane structure of the plasma-treated Candida albicans. Histological analysis showed that non-thermal plasma treatment did not cause epidermal damage or tissue inflammation and did not significantly modify the collagen layers of the mouse skin. CONCLUSIONS: The results of this study suggest that non-thermal atmospheric-pressure plasma might be safe and effective for clinical applications in the field of dentistry.
