In vivo targeting of ERGpotassiumchannels inmice and dogs by a positron-emitting analogue of fluoroclofilium.
- Author:
Sang Wook KIM
1
;
Seung Dae YANG
;
Byung Jae AHN
;
Jeong Hoon PARK
;
Dong Soo LEE
;
Guido GESSNER
;
Stefan H HEINEMANN
;
Wilhelm HERDERING
;
Kook Hyun YU
Author Information
1. Radiaton Application Research Division, Korea Atomic Energy Research Institute, 150, Dukjin-dong, Yuseong, Daejeon 305-353, Korea.
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
anti-arrhythmia agent;
clofilium;
dog;
ERG1 potassium channel;
heart;
positron-emission tomography
- MeSH:
Animals;
Anti-Arrhythmia Agents/pharmacokinetics/*pharmacology;
Cell Line;
Dogs;
Electrons;
Ether-A-Go-Go Potassium Channels/*antagonists & inhibitors;
Female;
Inhibitory Concentration 50;
Kidney/metabolism;
Mice;
Mice, Inbred BALB C;
Myocardium/metabolism;
*Positron-Emission Tomography;
Potassium Channel Blockers/pharmacokinetics/*pharmacology;
Quaternary Ammonium Compounds/pharmacokinetics/pharmacology;
Research Support, Non-U.S. Gov't;
Tissue Distribution;
Xenopus
- From:Experimental & Molecular Medicine
2005;37(4):269-275
- CountryRepublic of Korea
- Language:English
-
Abstract:
The antiarrhythmic clofilium is an efficient blocker of hERG1 potassium channels that are strongly expressed in the heart. Therefore, derivatives of clofilium that emit positrons might be useful tools for monitoring hERG1 channels in vivo. Fluoroclofilium (F-clofilium) was synthesized and its channel-blocking properties were determined for hERG1 and hEAG1 channels expressed in HEK 293 cells and in Xenopus oocytes. When applied extracellularly in the whole-cell patch-clamp configuration, F-cloflium exhibited a slower onset of block when compared with clofilium, presumably owing to its lower membrane permeability. When applied in the inside-out configuration at the intracellular membrane side, it blocked hEAG1 channels almost as efficiently as clofilium (IC50 1.37 nM and 0.83 nM, respectively). Similar results were obtained for hERG1, showing Fclofilium is a potent hERG1 and hEAG1 channel blocker once it has reached the intracellularly accessible target site at the channel. Using the 18Flabeled analog we studied the in vivo binding and distribution of F-clofilium in mice and a dog. Greatest activity was found in kidneys and bones. A small but significant enrichment of activity in the dog myocardium known for its expression of cERG1 channels allowed to depict the myocardium of a living dog by PET. Thus, F-clofilium is a useful tool for imaging hERG channels in living organisms.
