Development of an angiogenesis-focused cDNA chip and validation of its functionality.
- Author:
Joong Sup SHIM
1
;
Hae Kwang LEE
;
Hyo Mi PARK
;
Jong Ok KIM
;
Eun Kyung KIM
;
Kyung Hoon HWANG
;
Ki Tae KIM
;
Seong Whan PARK
;
Je Hyeon LEE
;
Ho Jeong KWON
Author Information
1. Chemical Genomics National Research Laboratory, Department of Bioscience and Biotechnology, Institute of Bioscience, Sejong University, 98 Kunja-dong, Kwangjin-gu, Seoul 143-747, Korea. kwonhj@sejong.ac.kr
- Publication Type:Case Report ; Research Support, Non-U.S. Gov't ; Validation Studies
- Keywords:
angiogenesis;
DNA chip;
duplicate spots, gene expression profile;
histone deacetylase
- MeSH:
Angiogenesis Inducing Agents/pharmacology;
Angiogenesis Inhibitors/pharmacology;
Gene Expression/drug effects;
Gene Expression Profiling/*instrumentation;
Humans;
Neovascularization, Pathologic/*genetics;
Neovascularization, Physiologic/*genetics;
Oligonucleotide Array Sequence Analysis/*instrumentation;
Research Support, Non-U.S. Gov't;
Tumor Cells, Cultured
- From:Experimental & Molecular Medicine
2005;37(4):365-370
- CountryRepublic of Korea
- Language:English
-
Abstract:
DNA chip has been used as a powerful tool to study the genetic reprogramming of cells and its link to cellular phenotype such as angiogenesis. To evaluate the angiogenesis related genetic reprogramming more efficiently, we here developed an angiogenesis- focused cDNA chip containing 153 angiogenesis related genes arrayed in duplicate on a slide glass. In order to validate the functionality of the angiogenesis-focused cDNA chip, we examined gene expression profiles in HT1080 cells treated with either fetal bovine serum, a well known pro-angiogenic factor, or trichostatin A, a known angiogenesis inhibitor, using the cDNA chip. All duplicate data from the analysis are well matched with each other and gene expression profiles are well consistent with previously reported data. These results demonstrate that the angiogenesis-focused cDNA chip developed here can be a useful tool towards angiogenesisrelated researches.
