Expression of Genetic Polymorphism of Interleukin-10 (IL-10) and Tumor Necrosis Factor-beta (TNF-beta) in Patients with Atopic Dermatitis.
- Author:
Bo Kyung KOH
1
;
Jung Soo KIM
;
Hoon HAN
;
Kweon Haeng LEE
;
Jin Wou KIM
Author Information
1. Department of Dermatology, College of Medicine, The Catholic University of Korea, Seoul, Korea. PAULDERMA@yahoo.co.kr
- Publication Type:Original Article
- Keywords:
Atopic dermatitis;
IL-10;
TNF-beta
- MeSH:
Antibody Formation;
Autoimmunity;
B-Lymphocytes;
Dermatitis, Atopic*;
Genotype;
Humans;
Immunoglobulin E;
Interleukin-10*;
Interleukin-13;
Interleukin-4;
Interleukin-5;
Lymphotoxin-alpha*;
Polymorphism, Genetic*;
Skin Diseases
- From:Korean Journal of Dermatology
2002;40(5):488-495
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Atopic dermatitis (AD) is a common inflammatory skin disease. Atopic dermatitis is associated with increased IL-4, IL-5, IL-10 and IL-13 but decreased INF-gamma and TNF production. IL-10 production has been implicated in autoimmunity because of its effect on B-cell proliferation and antibody production. The study of IL-10 gene polymorphism is of interest because of the pivotal role of IL-10 in the regulation of inflammatory and immune responses. TNF-beta significantly upregulates INF-gamma but downregulates IL-5, IL-13 and IgE, which suggests a potential role of TNF-beta in the pathogenesis of atopic dermatitis. OBJECTIVES: We have investigated polymorphism of IL-10 promoter gene and TNF-beta gene. METHODS: Seventy one patients with atopic dermatitis and one hundred and sixty six normal subjects participated in this study with analysis of polymorphism of IL-10 promoter (-1082), (-819) gene and seventy one patients with atopic dermatitis and one hundred and forty one normal subjects participated in the analysis of polymorphism of TNF-beta gene. The patients in this study were recently diagnosed with atopic dermatitis. RESULTS: The frequency of IL-10 promoter (-1082) genotypes (A/A, A/G, G/G), genes (A, G), IL-10 promoter (-819) genotypes (T/T, T/C, C/C) and genes (T, C) did not show any significant difference between atopic dermatitis patients and normal controls. There was no significant difference in the frequency of TNFB genotypes (TNFB*1/TNFB*1, TNFB*1/TNFB*2, TNFB*2/TNFB*2) and genes (TNFB*1, TNFB*2) in patients of atopic dermatitis and normal controls. CONCLUSION: The results of this study suggest that the other regions of the IL-10 promoter gene and TNFB gene should be investigated for polymorphism of atopic dermatitis. And the difference of IL-10 promoter and TNFB gene polymorphism between caucasian and Korean needs to be evaluated.
