Assessment of the Cytotoxic and Apoptotic Effects of Chaetominine in a Human Leukemia Cell Line.
10.4062/biomolther.2015.093
- Author:
Jingyun YAO
1
;
Ruihua JIAO
;
Changqing LIU
;
Yupeng ZHANG
;
Wanguo YU
;
Yanhua LU
;
Renxiang TAN
Author Information
1. State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, PR China. luyanhua@ecust.edu.cn
- Publication Type:Original Article
- Keywords:
Cytotoxic;
Apoptosis;
Mitochondrial pathway;
K562 cell
- MeSH:
Apoptosis;
Aspergillus fumigatus;
Caspase 3;
Caspase 9;
Cell Death;
Cell Line*;
Cytochromes c;
Cytosol;
DNA Fragmentation;
Fungi;
Humans*;
Inhibitory Concentration 50;
K562 Cells;
Leukemia*;
Membrane Potential, Mitochondrial;
Mitochondria
- From:Biomolecules & Therapeutics
2016;24(2):147-155
- CountryRepublic of Korea
- Language:English
-
Abstract:
Chaetominine is a quinazoline alkaloid originating from the endophytic fungus Aspergillus fumigatus CY018. In this study, we showed evidence that chaetominine has cytotoxic and apoptotic effects on human leukemia K562 cells and investigated the pathway involved in chaetominine-induced apoptosis in detail. Chaetominine inhibited K562 cell growth, with an IC50 value of 35 nM, but showed little inhibitory effect on the growth of human peripheral blood mononuclear cells. The high apoptosis rates, morphological apoptotic features, and DNA fragmentation caused by chaetominine indicated that the cytotoxicity was partially caused by its pro-apoptotic effect. Under chaetominine treatment, the Bax/Bcl-2 ratio was upregulated (from 0.3 to 8), which was followed by a decrease in mitochondrial membrane potential, release of cytochrome c from mitochondria into the cytosol, and stimulation of Apaf-1. Furthermore, activation of caspase-9 and caspase-3, which are the main executers of the apoptotic process, was observed. These results demonstrated that chaetominine induced cell apoptosis via the mitochondrial pathway. Chaetominine inhibited K562 cell growth and induced apoptotic cell death through the intrinsic pathway, which suggests that chaetominine might be a promising therapeutic for leukemia.
