Comparative Evaluation of Hepatoprotective Activities of Geniposide, Crocins and Crocetin by CCl4-Induced liver Injury in Mice.
10.4062/biomolther.2015.094
- Author:
Ping CHEN
1
;
Yang CHEN
;
Yarong WANG
;
Shining CAI
;
Liang DENG
;
Jia LIU
;
Hao ZHANG
Author Information
1. Department of Pharmacognosy, West China School of Pharmacy, Sichuan University, Chengdu 610041, PR China. zhanghhx@vip.sina.com
- Publication Type:Original Article
- Keywords:
Gardenia jasminoides Ellis;
Hepatoprotective;
Geniposide;
Crocin-1;
Crocetin;
Histopathological examination
- MeSH:
Alanine Transaminase;
Alkaline Phosphatase;
Animals;
Aspartate Aminotransferases;
Gardenia;
Glutathione;
Hepatocytes;
Iridoid Glycosides;
Liver*;
Mice*;
Rupture
- From:Biomolecules & Therapeutics
2016;24(2):156-162
- CountryRepublic of Korea
- Language:English
-
Abstract:
Iridoid glycosides (mainly geniposide) and crocetin derivatives (crocins) are the two major active constituents in Gardenia jasminoides Ellis. In the present study, geniposide, crocins, crocin-1 and crocetin were separated from gardenia chromatographically. Then, mice were orally administrated with geniposide (400 mg/kg b.w.), crocins (400 mg/kg b.w.), crocin-1 (400 mg/kg b.w.) and crocetin (140 mg/kg b.w.) once daily for 7 days with CCl4. Hepatoprotective properties were evaluated by biochemical parameters: Administration of geniposide, crocins, crocin-1and crocetin significantly lowered serum alanine transaminase (ALT), aspartate transaminase (AST) and alkaline phosphatase (ALP) levels in CCl4-treated mice. The reduced glutathione (GSH) levels and antioxidant enzymes (SOD and CAT) activities were also increased by geniposide, crocins, crocin-1 and crocetin. Histopathological examination of livers showed that these components reduced deformability, irregular arrangement and rupture of hepatocyte in CCl4-treated mice. These biochemical results and liver histopathological assessment demonstrated that geniposide, crocetin derivatives and crocetin show comparative beneficial effects on CCl4-induced liver damage via induction of antioxidant defense. Therefore, contents of geniposide and crocetin derivatives should be both considered for hepatoprotective efficacy of Gardenia jasminoides Ellis.
