Apigenin Regulates Interleukin-1β-Induced Production of Matrix Metalloproteinase Both in the Knee Joint of Rat and in Primary Cultured Articular Chondrocytes.
10.4062/biomolther.2015.217
- Author:
Jin Sung PARK
1
;
Dong Kyu KIM
;
Hyun Dae SHIN
;
Hyun Jae LEE
;
Ho Seung JO
;
Jin Hoon JEONG
;
Young Lac CHOI
;
Choong Jae LEE
;
Sun Chul HWANG
Author Information
1. Department of Orthopedic Surgery and Institute of Health Sciences, School of Medicine and Hospital, Gyeongsang National University, Jinju 52727, Republic of Korea. hscspine@hanmail.net
- Publication Type:In Vitro ; Original Article
- Keywords:
Apigenin;
Chondrocyte;
Metalloproteinase;
Osteoarthritis
- MeSH:
Animals;
Apigenin*;
Blotting, Western;
Caseins;
Chondrocytes*;
Gene Expression;
Knee Joint*;
Knee*;
Osteoarthritis;
Polymerase Chain Reaction;
Rats*;
Reverse Transcription;
Thrombospondins
- From:Biomolecules & Therapeutics
2016;24(2):163-170
- CountryRepublic of Korea
- Language:English
-
Abstract:
We examined whether apigenin affects the gene expression, secretion and activity of matrix metalloproteinase-3 (MMP-3) in primary cultured rabbit articular chondrocytes, as well as in vivo production of MMP-3 in the knee joint of rat to evaluate the potential chondroprotective effects of apigenin. Rabbit articular chondrocytes were cultured in a monolayer, and reverse transcription - polymerase chain reaction (RT-PCR) was used to measure interleukin-1β (IL-1β)-induced expression of MMP-3, MMP-1, MMP-13, a disintegrin and metalloproteinase with thrombospondin motifs-4 (ADAMTS-4), and ADAMTS-5. In rabbit articular chondrocytes, the effects of apigenin on IL-1β-induced secretion and proteolytic activity of MMP-3 were investigated using western blot analysis and casein zymography, respectively. The effect of apigenin on MMP-3 protein production was also examined in vivo. In rabbit articular chondrocytes, apigenin inhibited the gene expression of MMP-3, MMP-1, MMP-13, ADAMTS-4, and ADAMTS-5. Furthermore, apigenin inhibited the secretion and proteolytic activity of MMP-3 in vitro, and inhibited production of MMP-3 protein in vivo. These results suggest that apigenin can regulate the gene expression, secretion, and activity of MMP-3, by directly acting on articular chondrocytes.
