Methods of Hematoxylin and Erosin Image Information Acquisition and Optimization in Confocal Microscopy.
10.4258/hir.2016.22.3.238
- Author:
Woong Bae YOON
1
;
Hyunjin KIM
;
Kwang Gi KIM
;
Yongdoo CHOI
;
Hee Jin CHANG
;
Dae Kyung SOHN
Author Information
1. Biomedical Engineering Branch, Research Institute and Hospital, National Cancer Center, Goyang, Korea. gsgsbal@ncc.re.kr
- Publication Type:Case Report
- Keywords:
Confocal Laser Scanning Microscopy;
Computer-Assisted Image Processing;
Fluorescence;
Histological Labeling;
Hematoxylin
- MeSH:
Diagnosis;
Eosine Yellowish-(YS);
Fluorescence;
Hematoxylin*;
Image Processing, Computer-Assisted;
Methods*;
Microscopy;
Microscopy, Confocal*;
Staining and Labeling
- From:Healthcare Informatics Research
2016;22(3):238-242
- CountryRepublic of Korea
- Language:English
-
Abstract:
OBJECTIVES: We produced hematoxylin and eosin (H&E) staining-like color images by using confocal laser scanning microscopy (CLSM), which can obtain the same or more information in comparison to conventional tissue staining. METHODS: We improved images by using several image converting techniques, including morphological methods, color space conversion methods, and segmentation methods. RESULTS: An image obtained after image processing showed coloring very similar to that in images produced by H&E staining, and it is advantageous to conduct analysis through fluorescent dye imaging and microscopy rather than analysis based on single microscopic imaging. CONCLUSIONS: The colors used in CLSM are different from those seen in H&E staining, which is the method most widely used for pathologic diagnosis and is familiar to pathologists. Computer technology can facilitate the conversion of images by CLSM to be very similar to H&E staining images. We believe that the technique used in this study has great potential for application in clinical tissue analysis.
