Effects of Intracoronary Administration of Autologous Adipose Tissue-Derived Stem Cells on Acute Myocardial Infarction in a Porcine Model.
10.3349/ymj.2015.56.6.1522
- Author:
Hye Won LEE
1
;
Han Cheol LEE
;
Jong Ha PARK
;
Bo Won KIM
;
Jinhee AHN
;
Jin Hee KIM
;
Jin Sup PARK
;
Jun Hyok OH
;
Jung Hyun CHOI
;
Kwang Soo CHA
;
Taek Jong HONG
;
Tae Sik PARK
;
Sang Pil KIM
;
Seunghwan SONG
;
Ji Yeon KIM
;
Mi Hwa PARK
;
Jin Sup JUNG
Author Information
1. Division of Cardiology, Pusan National University Hospital, Busan, Korea. glaraone@hanmail.net
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Myocardial infarction;
angiogenesis;
mesenchymal stem cells;
left ventricle remodeling;
99mTc MIBI
- MeSH:
Adipose Tissue/cytology;
Animals;
Bone Marrow Cells/cytology/*metabolism;
Chemokine CXCL12;
Coronary Vessels;
Female;
Heart/physiopathology;
Heart Ventricles;
*Mesenchymal Stromal Cells;
Myocardial Infarction/physiopathology/radionuclide imaging/*therapy;
*Stem Cell Transplantation;
Swine;
Technetium Tc 99m Sestamibi/*pharmacology;
Tomography, Emission-Computed, Single-Photon/*methods;
Troponin T;
*Ventricular Function, Left
- From:Yonsei Medical Journal
2015;56(6):1522-1529
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: Adipose-derived stem cells (ADSCs) are known to be potentially effective in regeneration of damaged tissue. We aimed to assess the effectiveness of intracoronary administration of ADSCs in reducing the infarction area and improving function after acute transmural myocardial infarction (MI) in a porcine model. MATERIALS AND METHODS: ADSCs were obtained from each pig's abdominal subcutaneous fat tissue by simple liposuction. After 3 passages of 14-days culture, 2 million ADSCs were injected into the coronary artery 30 min after acute transmural MI. At baseline and 4 weeks after the ADSC injection, 99mTc methoxyisobutylisonitrile-single photon emission computed tomography (MIBISPECT) was performed to evaluate the left ventricular volume, left ventricular ejection fraction (LVEF; %), and perfusion defects as well as the myocardial salvage (%) and salvage index. At 4 weeks, each pig was sacrificed, and the heart was extracted and dissected. Gross and microscopic analyses with specific immunohistochemistry staining were then performed. RESULTS: Analysis showed improvement in the perfusion defect, but not in the LVEF in the ADSC group (n=14), compared with the control group (n=14) (perfusion defect, -13.0+/-10.0 vs. -2.6+/-12.0, p=0.019; LVEF, -8.0+/-15.4 vs. -15.9+/-14.8, p=0.181). There was a tendency of reducing left ventricular volume in ADSC group. The ADSCs identified by stromal cell-derived factor-1 (SDF-1) staining were well co-localized by von Willebrand factor and Troponin T staining. CONCLUSION: Intracoronary injection of cultured ADSCs improved myocardial perfusion in this porcine acute transmural MI model.
