Immunization effect of recombinant P27/30 protein expressed in Escherichia coli against the hard tick Haemaphysalis longicornis (Acari: Ixodidae) in rabbits.
10.3347/kjp.2004.42.4.195
- Author:
Myung Jo YOU
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
P27/30 protein;
Haemaphysalis longicornis;
ticks;
immunity;
tick control
- MeSH:
Animals;
Antibodies/blood;
Escherichia coli/genetics;
Female;
Gene Expression;
Insect Proteins/immunology;
Ixodidae/*immunology;
Microfilament Proteins/*immunology;
Rabbits;
Recombinant Proteins/*immunology;
Research Support, Non-U.S. Gov't;
Tick Infestations/*immunology/prevention & control
- From:The Korean Journal of Parasitology
2004;42(4):195-200
- CountryRepublic of Korea
- Language:English
-
Abstract:
We investigated the induction of resistance to Haemaphysalis longicornis infestation in rabbits that had been immunized with recombinant H. longicornis P27/30 protein. The success of immunological control methods is dependent upon the use of potential key antigens as tick vaccine candidates. Previously, we cloned a gene encoding 27 kDa and 30 kDa proteins (P27/30) of H. longicornis, and identified P27/30 as a troponin I-like protein. In this study, rabbits that were immunized with recombinant P27/30 expressed in Escherichia coli showed the statistically significant longer feeding duration for larval and adult ticks (P< 0.05), low engorgement rates in larval ticks (64.4%), and an apparent reduction in egg weights, which suggest that H. longicornis P27/30 protein is a potential candidate antigen for a tick vaccine. These results demonstrated that the recombinant P27/30 protein might be a useful vaccine candidate antigen for biological control of H. longicornis.
