Efficacy of Dendritic Cells Matured Early with OK-432 (Picibanil(R)), Prostaglandin E2, and Interferon-alpha as a Vaccine for a Hormone Refractory Prostate Cancer Cell Line.
10.3346/jkms.2010.25.9.1284
- Author:
Changhee YOO
1
;
Hyun Ah DO
;
In Gab JEONG
;
Hongzoo PARK
;
Jung Jin HWANG
;
Jun Hyuk HONG
;
Jin Seon CHO
;
Myong Soo CHOO
;
Hanjong AHN
;
Choung Soo KIM
Author Information
1. Department of Urology, College of Medicine, Hallym University, Hallym Sacred Heart Hospital, Anyang, Korea.
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Dendritic Cells;
Prostatic Neoplasms;
Cancer Vaccines;
Immunotherapy
- MeSH:
Cancer Vaccines/*immunology;
Cell Line, Tumor;
Dendritic Cells/cytology/drug effects/*immunology;
Dinoprostone/*pharmacology;
Humans;
Immunologic Factors/*pharmacology;
Interferon-alpha/*pharmacology;
Lipopolysaccharides/toxicity;
Male;
Neoplasms, Hormone-Dependent/*immunology;
Phenotype;
Picibanil/*pharmacology;
Prostatic Neoplasms/*immunology;
T-Lymphocytes, Cytotoxic/immunology
- From:Journal of Korean Medical Science
2010;25(9):1284-1290
- CountryRepublic of Korea
- Language:English
-
Abstract:
Dendritic cells (DCs) are potent antigen-presenting cells. OK432 (Picibanil(R)) was introduced as a potent stimulator of DC maturation in combination with prostaglandin-E2 and interferon-alpha. We compared the efficacy of a DC-prostate cancer vaccine using early-mature DCs stimulated with OK432, PGE2 and INF-alpha (OPA) with that of vaccines using other methods. On days 3 or 7 of DC culture, TNF-alpha (T), TNF-alpha and LPS (TL) or OPA were employed as maturation stimulators. DU145 cells subjected to heat stress were hybridized with mature DCs using polyethyleneglycol. T cells were sensitized by the hybrids, and their proliferative and cytokine secretion activities and cytotoxicity were measured. The yields of early-mature DCs were higher, compared to yields at the conventional maturation time (P<0.05). In the early maturation setting, the mean fusion ratios, calculated from the fraction of dual-positive cells, were 13.3%, 18.6%, and 39.9%, respectively (P=0.051) in the T only, TL, and OPA-treated groups. The function of cytotoxic T cells, which were sensitized with the hybrids containing DCs matured early with OPA, was superior to that using other methods. The antitumor effects of DC-DU145 hybrids generated with DCs subjected to early maturation with the OPA may be superior to that of the hybrids using conventional maturation methods.
