An oral Aujeszky's disease vaccine (YS-400) induces neutralizing antibody in pigs.
10.7774/cevr.2016.5.2.132
- Author:
Dong Kun YANG
1
;
Ha Hyun KIM
;
Sung Suk CHOI
;
Bang Hun HYUN
;
Jae Young SONG
Author Information
1. Viral Disease Division, Animal and Plant Quarantine Agency, MAFRA, Gimcheon, Korea. yangdk@korea.kr
- Publication Type:Original Article
- Keywords:
Aujeszky's disease virus;
Immunity;
Oral vaccine
- MeSH:
Animals;
Antibodies, Neutralizing*;
Blister;
Disease Outbreaks;
Enzyme-Linked Immunosorbent Assay;
Herpesvirus 1, Suid;
Pseudorabies*;
Sus scrofa;
Swine*;
Syringes;
Vaccination;
Vero Cells
- From:Clinical and Experimental Vaccine Research
2016;5(2):132-137
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: Aujeszky's disease (AD) is an economically important disease affecting both wild and domestic pigs of the species Sus scrofa. A previous study yielded serological evidence of AD in Korean wild boars, which could spread AD to other animals. A new Aujeszky's disease virus (ADV) bait vaccine is required to prevent AD outbreaks in swine. In the present study, we investigated the safety and immunogenicity of a gE-deleted marker vaccine, strain YS-400, in young domestic pigs. MATERIALS AND METHODS: The YS-400 strain was propagated in Vero cells, and the trial ADV bait vaccine (a vaccine blister in a matrix including an attractant) was prepared. Pigs were orally immunized with the vaccine (2 mL, 10(7.5) TCID(50)/mL) delivered using a syringe or in the bait vaccine. The animals were observed for 9 weeks after vaccination, and immunogenicity was assessed using a virus neutralization (VN) test and enzyme linked immunosorbent assay. RESULTS: The YS-400 strain was non-pathogenic to pigs when given orally and induced high VN titers (1:32-1:128) 6 weeks post-administration. Of the pigs given the ADV bait vaccine twice or three times, 40% were seropositive by 2 weeks, and 100% were seropositive by 7 weeks after the first dose. Pigs that consumed the AD bait vaccine three times developed VN titers that were slightly higher than those of pigs given the vaccine twice. CONCLUSION: Domestic pigs given the trial ADV bait vaccine exhibited no adverse effects and developed high VN titers against ADV, indicating that the YS-400 strain is safe and can prevent ADV infection in domestic pigs.
