Safety and immunogenicity of recombinant rabies virus (ERAGS) in mice and raccoon dogs.
10.7774/cevr.2016.5.2.159
- Author:
Dong Kun YANG
1
;
Ha Hyun KIM
;
Sung Suk CHOI
;
Jong Tack KIM
;
Kang Bok LEE
;
Seong Heon LEE
;
In Soo CHO
Author Information
1. Viral Disease Division, Animal and Plant Quarantine Agency, MAFRA, Gimcheon, Korea. yangdk@korea.kr
- Publication Type:Original Article
- Keywords:
Rabies virus;
Mouth;
Vaccines;
Raccoon dogs
- MeSH:
Animals;
Glycoproteins;
Korea;
Mice*;
Mouth;
Mutagenesis, Site-Directed;
Neuroblastoma;
Neutralization Tests;
Rabies Vaccines;
Rabies virus*;
Rabies*;
Raccoon Dogs*;
Raccoons*;
Vaccines
- From:Clinical and Experimental Vaccine Research
2016;5(2):159-168
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: The development of a genetically modified live rabies vaccine applicable to wild raccoon dogs is necessary for the eradication of rabies in Korea. Thus, we constructed a recombinant rabies virus (RABV) called the ERAGS strain, using a reverse genetic system and evaluated its safety and efficacy in mice and its safety and immunogenicity in raccoon dogs. MATERIALS AND METHODS: ERAGS, which has Asn194Ser and Arg333Glu substitutions in the glycoprotein, was constructed using site-directed mutagenesis. Mice were inoculated with the ERAGS strain (either 10(5.0) or 10(7.0) FAID(50)/mL) via intramuscular (IM) or intracranial injections and then challenged with a virulent RABV. Raccoon dogs were administered the ERAGS strain (10(8.0) FAID(50)/mL) either orally or via the IM route and the immunogenicity of the strain was evaluated using fluorescent antibody virus neutralization tests. RESULTS: The ERAGS strain inoculated into murine neuroblastoma cells reached 10(7.8) FAID(50)/mL at 96-hour post-inoculation. The virus was not pathogenic and induced complete protection from virulent RABV in immunized 4- and 6-week-old mice. Korean raccoon dogs immunized with the ERAGS strain via IM or oral route were also safe from the virus and developed high titer levels (26.4-32.8 IU/mL) of virus-neutralizing antibody (VNA) at 4 weeks post-inoculation. CONCLUSION: The ERAGS RABV strain was effectively protective against rabies in mice and produced a high VNA titer in raccoon dogs.
