A Bordetella pertussis proteoliposome induces protection in mice without affecting the immunogenicity of diphtheria and tetanus toxoids in a trivalent formulation.
10.7774/cevr.2016.5.2.175
- Author:
Sonsire Fernández CASTILLO
1
;
Mario Landys CHOVEL
;
Niurka Gutiérrez HERNÁNDEZ
;
Lorena Corcho GONZÁLEZ
;
Amaya BLANCO
;
Daily Serrano HERNÁNDEZ
;
Mildrey Fariñas MEDINA
;
Maydelis Alvarez TITO
;
José Luis Pérez QUIÑOY
Author Information
1. Finlay Institute, Havana, Cuba. sfernandez@finlay.edu.cu
- Publication Type:Brief Communication
- Keywords:
Bordetella pertussis;
Proteoliposome;
Whooping cough;
Vaccines
- MeSH:
Animals;
Bordetella pertussis*;
Bordetella*;
Diphtheria Toxoid;
Diphtheria*;
Enzyme-Linked Immunosorbent Assay;
Lung;
Mice*;
Neutralization Tests;
Tetanus Toxoid*;
Tetanus*;
Vaccines;
Whooping Cough
- From:Clinical and Experimental Vaccine Research
2016;5(2):175-178
- CountryRepublic of Korea
- Language:English
-
Abstract:
In this study, a formulation of Bordetella pertussis proteoliposome (PLBp), diphtheria, and tetanus toxoids and alum (DT-PLBp) was evaluated as a trivalent vaccine candidate in BALB/c mice. Vaccine-induced protection was estimated using the intranasal challenge for pertussis and enzyme-linked immunosorbent assay fvto assess serological responses for diphtheria or tetanus. Both, diphtheria-tetanus-whole cell pertussis (DTP) and diphtheria-tetanus vaccines (DT) were used as controls. Animals immunized with DT-PLBp, PLBp alone, and DTP showed total reduction of CFU in lungs 7 days after intranasal challenge. Likewise, formulations DT-PLBp, DTP, and DT elicited antibody levels ≥2 IU/mL against tetanus and diphtheria, considered protective when neutralization tests are used. Overall, results showed that combination of PLBp with tetanus and diphtheria toxoids did not affect the immunogenicity of each antigen alone.
