Significance of Serology by Multi-Antigen ELISA for Tissue Helminthiases in Korea.
10.3346/jkms.2017.32.7.1118
- Author:
Yan JIN
1
;
Eun Min KIM
;
Min Ho CHOI
;
Myoung Don OH
;
Sung Tae HONG
Author Information
1. Department of Microbiology, Dongguk University College of Medicine, Gyeongju, Korea.
- Publication Type:Original Article
- Keywords:
Tissue Helminthiases;
Seropositivity;
IgG Antibody;
ELISA;
Cross-Reaction;
Multiple Infections;
Korea
- MeSH:
Antibodies;
Antigens, Helminth;
Clonorchiasis;
Cysticercosis;
Diagnosis;
Enzyme-Linked Immunosorbent Assay*;
Female;
Helminthiasis*;
Hospital Records;
Humans;
Immunoglobulin G;
Korea*;
Male;
Paragonimiasis;
Prevalence;
Serologic Tests;
Sparganosis
- From:Journal of Korean Medical Science
2017;32(7):1118-1123
- CountryRepublic of Korea
- Language:English
-
Abstract:
It is clinically important to differentiate tissue-invading helminthiasis. The purpose of this study was to assess the specific immunoglobulin G (IgG) antibody positive rates for clonorchiasis, paragonimiasis, cysticercosis, and sparganosis 4 helminthiases from 1996 to 2006 using multi-antigen enzyme-linked immunosorbent assay (ELISA) in Korea. Results of 6,017 samples, which were referred to our institute for serodiagnosis, were analyzed. The subjects with positive serum IgG antibodies were 1,502 (25.0%) for any of the 4 helminthiases. The overall positive numbers for clonorchiasis, paragonimiasis, cysticercosis, and sparganosis were 728 (12.1%), 166 (2.8%), 729 (12.1%), and 263 (4.4%), respectively. The positive serologic reaction to multi-antigens was determined in 309 (20.6%) of the 1,502 total seropositive subjects. Those with multi-antigen positivity were regarded as positive for the antigen of strongest reaction but cross-reaction to others with weak positive reaction. Annual seropositive rates for those 4 tissue helminthiases ranged from 12.1% to 35.7%. The highest rate was observed in age from 60 to 69 years old and prevalence of men (27.4%; 1,030/3,763) was significantly higher than of women (19.1%; 332/1,741). Hospital records of 165 ELISA positive patients were reviewed to confirm correlation with their clinical diagnosis. Paragonimiasis was highly correlated as 81.8% (9/11), cysticercosis 29.9% (20/67), clonorchiasis 29.0% (20/69), and sparganosis 11.1% (2/18). In conclusion, the multi-antigen ELISA using 4 helminth antigens is useful to differentiate suspected tissue-invading helminthiases, especially ELISA diagnosis of paragonimiasis is reliable. The seropositivity is still high among suspected patients in Korea.
