Attenuation of endothelial relaxation in umbilical arteries from preeclampsia patients.
- Author:
Sei Kwang KIM
;
Hyung Min CHOI
;
Yong Won PARK
;
Jae Wook KIM
;
Duck Sun AHN
;
Bok Soon KANG
- Publication Type:Original Article
- Keywords:
Preeclampsia;
Umbilical artery;
Vasoactive substance;
Endothelial cell dependent relaxation(EDR);
Endothelial cell independent relaxation
- MeSH:
Biological Assay;
Bradykinin;
Calcimycin;
Endothelial Cells;
Femoral Artery;
Humans;
Muscle, Smooth;
Myocytes, Smooth Muscle;
Pre-Eclampsia*;
Relaxation*;
Tissue Donors;
Umbilical Arteries*;
Vascular Resistance
- From:Korean Journal of Obstetrics and Gynecology
2000;43(6):1043-1050
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVES AND METHODS: To directly examine the function of the endothelial cell(EC) and smooth muscle cell in umbilical arteries acquired from preeclampsia patients between June 1998 to November 1999, using a conventional tension measurement and bioassay experiment. RESULTS: Relaxation responses to EC-dependent relaxing agents including bradykinin and A23187 in human umbilical artery rings were significantly decreased in preeclampsia(p<0.01). Relaxation responses to EC-independent agents(SNP and SNAP) were also inhibited in umbilical artery rings acquired from preeclampsia patients(p<0.01). To test the change of endothelial cell function in preeclampsia without involvement of smooth muscle dysfunction, we used human umbilical artery and rabbit femoral artery as a donor and detector, respectively, in bioassay experiment. Relaxation responses to EC-dependent agents(A23187 and bradykinin) showed similar results to conventional tension measurement (p<0.01). Relaxation responses to 8-bromo-cGMP in human umbilical artery rings were also significantly decreased in preeclampsia(p<0.01). CONCLUSIONS: It can be concluded that increased vascular resistance in preeclampsia is not only due to the disturbance of endothelial function, but also to smooth muscle dysfunction.
