Effects of d-alpha-tocopherol supplements on lipid metabolism in a high-fat diet-fed animal model.
- Author:
Do Yeon KIM
1
;
Jinkyung KIM
;
Hye Jin HAM
;
Ryowon CHOUE
Author Information
- Publication Type:Original Article
- Keywords: High fat diet; supplementation; d-alpha-tocopherol; insulin resistance
- MeSH: Administration, Oral; Animals*; Cholesterol; Diet; Diet, High-Fat; Humans; Insulin Resistance; Lipid Metabolism*; Lipid Peroxidation; Liver; Male; Malondialdehyde; Mice; Models, Animal*; Oxidative Stress; Peroxisomes; Triglycerides; Vitamin E; Vitamins
- From:Nutrition Research and Practice 2013;7(6):481-487
- CountryRepublic of Korea
- Language:English
- Abstract: High-fat diet up-regulates either insulin resistance or triglycerides, which is assumed to be related to the expression of peroxisome proliferator-activated receptor (PPAR)-alpha and PPAR-gamma. The beneficial effects of vitamin E on insulin resistance are well known; however, it is not clear if vitamin E with a high-fat diet alters the expression of PPAR-alpha and PPAR-gamma. We investigated the effects of d-alpha-tocopherol supplementation on insulin sensitivity, blood lipid profiles, lipid peroxidation, and the expression of PPAR-alpha and PPAR-gamma in a high-fat (HF) diet-fed male C57BL/6J model of insulin resistance. The animals were given a regular diet (CON; 10% fat), a HF diet containing 45% fat, or a HF diet plus d-alpha-tocopherol (HF-E) for a period of 20 weeks. The results showed that the HF diet induced insulin resistance and altered the lipid profile, specifically the triglyceride (TG) and total cholesterol (TC) levels (P < 0.05). In this animal model, supplementation with d-alpha-tocopherol improved insulin resistance as well as the serum levels of TG and very-low-density lipoprotein-cholesterol (VLDL-C) (P < 0.05). Moreover, the treatment decreased the levels of malondialdehyde (MDA) in the serum and liver while increasing hepatic PPAR-alpha expression and decreasing PPAR-gamma expression. In conclusion, the oral administration of d-alpha-tocopherol with a high-fat diet had positive effects on insulin resistance, lipid profiles, and oxidative stress through the expression of PPAR-alpha and PPAR-gamma in a high-fat diet-fed male mice.
