Identification of Brucella abortus using the sequencing of omp gene.
- Author:
Yu Ji LEE
1
;
Kwan Soo KO
;
Mi Yeoun PARK
;
Won Sup OH
;
Ki Tae KWON
;
Seong Yeol RYU
;
Sang Taek HEO
;
Kyong Ran PECK
;
Nam Yong LEE
;
Jae Hoon SONG
Author Information
1. Department of Internal Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea. wsoh@smc.samsung.co.kr
- Publication Type:Original Article
- Keywords:
Brucellosis;
Brucella arbortus;
16S ribosomal RNA;
omp2
- MeSH:
Animals;
Base Sequence;
Brucella abortus*;
Brucella*;
Brucellosis;
Brucellosis, Bovine;
Cattle;
Databases, Nucleic Acid;
DNA;
Fever;
Humans;
Incidence;
Korea;
Polymerase Chain Reaction;
RNA, Ribosomal, 16S;
Sequence Analysis, DNA
- From:Korean Journal of Medicine
2006;71(1):10-16
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: As the incidence of bovine brucellosis increases in Korea, the incidence of human brucellosis is also increasing since 2002. However, it is difficult to identify Brucella species by using the conventional methods. METHODS: Three strains of gram-negative coccobacilli were isolated from blood specimens of three patients with prolonged fever, which were not identified by using the conventional methods. After extracting total DNA from these isolates, PCR amplification of 16S rRNA and omp2 genes was performed. These sequences secured by PCR assay were compared with known sequences by using GenBank BLAST. RESULTS: DNA sequences were obtained from 3 isolates by using PCR amplification of 16S rRNA. These sequences had more than 99.9% similarities with Brucella species by using GenBank BLAST. In the second place, after comparing DNA sequences secured by PCR amplification of omp2a and omp2b by using GenBank BLAST, these isolates were confirmed as B. abortus. CONCLUSIONS: DNA sequence analysis is a rapid and accurate method for identification of uncommon microorganisms, such as Brucella species.
