Collection, procesing and cryopreservation prior to freezing of cord blood
- Author:
Hieu Minh Tran
- Publication Type:Journal Article
- Keywords:
cord blood
- MeSH:
Fetal Blood;
Freezing
- From:Journal of Vietnamese Medicine
2002;269(2):1-6
- CountryViet Nam
- Language:Vietnamese
-
Abstract:
At the Hematology and Blood Transfusion Center Ho Chi Minh city. We have recently been performing collection, processing, and cryoprotection prior to freezing of cord blood for the first cord blood transplantation in Vietnam. The cord blood cells are sedimended by rouleaux formation using hydroxyethyl starch by gently centrifugation. The leukocyte-rich supematant is then transferred to a disposable processing bag set. The cells are then concentrated by a second centrifugation and removal of excess plasma supematant. The cryoprotection agent dimethyl sulfoxide is then slowly added to the concentrated cells, and the mixture transferred to a Themogenesis Corp, approved freezing bag for freezing and storage in the BioArchive System
