The Effect of the Transcriptional Regulation of Sp1 for TGF-beta1 and CTGF Expression in Scar Formation.
- Author:
Dong Man PARK
1
;
Dae Gu SOHN
;
Ki Hwan HAN
;
Sun Young LEE
;
Young Mi CHAE
;
Young Chae CHANG
;
Kwan Kyu PARK
Author Information
1. Department of Plastic and Reconstructive Surgery, Keimyung University School of Medicine, Daegu, Korea.
- Publication Type:Original Article
- Keywords:
Scar Formation;
Sp1 decoy oligodeoxynucleotide (ODN);
Transforming growth factor(TGF)-beta1;
Connective tissue growth factor(CTGF)
- MeSH:
Animals;
Cicatrix*;
Clone Cells;
Extracellular Matrix;
Fibroblasts;
Fibrosis;
Gene Expression;
Rats;
RNA;
Skin;
Transforming Growth Factor beta1*;
Wound Healing;
Wounds and Injuries
- From:Journal of the Korean Society of Plastic and Reconstructive Surgeons
2006;33(1):39-45
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
This study is to examine the relationship between TGF-b1 expression and CTGF expression, and to evaluate the effect of Sp1 blockade on the expression of TGF-b1, CTGF and extracellular genes, clones of fibroblasts stably transfected with Sp1 decoy ODN. R-Sp1 decoy ODN was highly resistant to degradation by nucleases or serum, compared to the linear or phosphorothioated-Sp1 decoy ODN. Skin wounds were created on the back of 36 anesthetized rats. They were divided into four groups-the rats with normal skin, with wounded skin without decoy, with wounded skin injected with R-Sp1 decoy, and with wounded skin injected with mismatched R-Sp1 decoy, respectively. Skins were collected at 3rd, 5th, 7th, 14th day after wounding. Cellular RNA was extracted by RT-PCR analysis. TGF-beta1 and CTGF were deeply related with skin fibrosis during scar formation and it appeared that TGF-beta1 may cause the induction of CTGF expression. R-Sp1 decoy ODN inhibited TGF-beta1 and CTGF expression both in cultured fibroblasts and in the skin of rats. These results indicate that targeting Sp1 with R-type decoy efficiently blocks extracellular matrix gene expression, and suggest an important new therapeutic approach to control the scarring in normal wound healing and fibrotic disorders.
