Polymorphisms of PPARgamma2 gene in patients with type 2 diabetes mellitus and obesity.
- Author:
Seung Joon OH
1
;
Sung Yi KANG
;
Young Seol KIM
;
Deog Yoon KIM
;
Sung Woon KIM
;
In Myung YANG
;
Jin Woo KIM
;
Young Kil CHOI
;
He Soon PARK
;
Jung Ryung PAENG
Author Information
1. Department of Internal Medicine, Kyung Hee University Medical School, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Liver cirrhosis;
Biliary;
Antimitochondrial antibody;
Anti-M2 antibody;
Enzyme-linked Immunosorbent assay
- MeSH:
Adipocytes;
Cholesterol;
Codon;
Diabetes Mellitus, Type 2*;
Enzyme-Linked Immunosorbent Assay;
Fasting;
Gene Frequency;
Genetic Variation;
Genotype;
Glucose;
Homozygote;
Humans;
Liver Cirrhosis;
Mass Screening;
Mutation, Missense;
Obesity*;
Peroxisomes;
PPAR gamma*;
Proline;
Triglycerides
- From:Korean Journal of Medicine
2000;59(2):132-141
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Peroxisome proliferator activated receptor-gamma (PPAR-gamma) is a nuclear receptor that regulate adipocyte differentiation and modulate intracellular insulin-signaling events. As such, PPARgamma is a candidate gene for several human disorders including obesity and type 2 diabetes mellitus. The objective of our study was to examine the relationship between genetic variation of PPARgamma2 and diabetes and obesity in Korean subjects. METHODS: We studied 99 subjects with type 2 diabetes mellitus, 128 obesity patients and 97 controls. Screening for mutation at codon 12 and 115 of PPARgamma2 were carried out by PCR-RFLP analyses. Statistical significance was evaluated by Chi-square test. RESULTS: The allele frequency of the Pro12Ala PPARgamma2 variant were 0.05 in controls, 0.06 in type 2 diabetes group, and 0.07 in obesity group (p=0.47). Pro115Gln variant were only proline homozygote in all groups. Genotype frequencies were also similar and conformed to expectations of the Hardy-Weinberg rule. The presence of PPARgamma2 gene variant was no associated with concentrations of total cholesterol, triglyceride, HDL-cholesterol, and also with fasting glucose. CONCLUSION: We concluded that the Pro12Ala and Pro115Gln PPARgamma2 missense mutation may not be associated with type 2 diabetes mellitus and obesity in Korean patients.
