Ureaplasma Contamination Rate in Donated Cord Blood Units .
- Author:
Eun Youn ROH
1
;
Sue SHIN
;
Jong Hyun YOON
;
Jee Young CHANG
Author Information
1. Department of Laboratory Medicine, Seoul National University Boramae Hospital, Seoul, Korea. crimson75@gmail.com
- Publication Type:Original Article
- Keywords:
Donated cord blood;
Ureaplasma;
Contamination
- MeSH:
Cell Culture Techniques;
DNA;
Fetal Blood;
Informed Consent;
Mycoplasma;
Polymerase Chain Reaction;
Ureaplasma
- From:Korean Journal of Blood Transfusion
2008;19(3):239-244
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Ureaplasma is one of the most common microorganisms in the genital tract, and also one of the most important contaminants in cell culture laboratories and cell banks. We analyzed the Ureaplasma contamination rate in donated cord blood units (CBUs) before cell processing. METHODS: One hundred fifty-one donated CBUs with informed consent collected between 3 November and 28 December 2006 were randomly selected and enrolled for study. All of the CBUs were obtained from full-term (37~42 weeks gestation) deliveries. We performed blood cultures and Ureaplasma DNA PCR assays using samples from the collection bags before processing. RESULTS: Two CBUs had positive blood culture results; however, Ureaplasma DNA was not found in the samples tested. CONCLUSION: The contamination rate of Ureaplasma in the donated CBUs from full-term deliveries without gestational and/or perinatal complications was extremely low. With the results of our previous Mycoplasma contamination assay (0%), donated CBUs can be used in culture and expansion processes without concerns for pre-processing mollicute contamination.
