Formulation and Characterization of Antigen-loaded PLGA Nanoparticles for Efficient Cross-priming of the Antigen.
- Author:
Young Ran LEE
1
;
Young Hee LEE
;
Sun A IM
;
Kyungjae KIM
;
Chong Kil LEE
Author Information
- Publication Type:Original Article
- Keywords: PLGA; Nanoparticle; Opsonization; Cross-priming
- MeSH: Animals; Cross-Priming; Dendritic Cells; Fluorescein; Immunoglobulin G; Isothiocyanates; Lactic Acid; Lymph Nodes; Macrophages; Mice; Nanoparticles; Ovalbumin; Ovum; Peptides; Phagocytes; Polyglycolic Acid; Polymers; Spleen
- From:Immune Network 2011;11(3):163-168
- CountryRepublic of Korea
- Language:English
- Abstract: BACKGROUND: Nanoparticles (NPs) prepared from biodegradable polymers, such as poly (D,L-lactic acid-co-glycolic acid) (PLGA), have been studied as vehicles for the delivery of antigens to phagocytes. This paper describes the preparation of antigen-loaded PLGA-NPs for efficient cross-priming. METHODS: NPs containing a similar amount of ovalbumin (OVA) but different sizes were produced using a micromixer-based W/O/W solvent evaporation procedure, and the efficiency of the NPs to induce the cross-presentation of OVA peptides were examined in dendritic cells (DCs). Cellular uptake and biodistribution studies were performed using fluorescein isothiocyanate (FITC)-loaded NPs in mice. RESULTS: The NPs in the range of 1.1~1.4microm in size were the most and almost equally efficient in inducing the cross-presentation of OVA peptides via H-2Kb molecules. Cellular uptake and biodistribution studies showed that opsonization of the NPs with mouse IgG greatly increased the percentage of FITC-positive cells in the spleen and lymph nodes. The major cell type of FITC-positive cells in the spleen was macrophages, whereas that of lymph nodes was DCs. CONCLUSION: These results show that IgG-opsonized PLGA-NPs with a mean size of 1.1microm would be the choice of biodegradable carriers for the targeted-delivery of protein antigens for cross-priming in vivo.
