The Role of Plasmacytoid and Myeloid Dendritic Cells in Induction of Asthma in a Mouse Model and the Effect of a TLR9 Agonist on Dendritic Cells.
10.4168/aair.2011.3.3.199
- Author:
Ji Hun MO
1
;
Young Jun CHUNG
;
Tomoko HAYASHI
;
Jongdae LEE
;
Eyal RAZ
Author Information
1. Department of Otorhinolaryngology-Head and Neck Surgery, Dankook University College of Medicine, Cheonan, Korea. jihunmo@gmail.com
- Publication Type:Original Article
- Keywords:
Plasmacytoid dendritic cell;
myeloid dendritic cell;
allergy;
immunostimulatory sequence;
TLR9;
migration
- MeSH:
Animals;
Asthma;
Bronchoalveolar Lavage;
Cell Count;
Dendritic Cells;
Eosinophils;
Hypersensitivity;
Lung;
Mice;
Respiratory Function Tests;
T-Lymphocytes
- From:Allergy, Asthma & Immunology Research
2011;3(3):199-204
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: To determine the role of plasmacytoid dendritic cells (pDC) and myeloid dendritic cells (mDC) in priming effector T cells to induce allergy, and to evaluate the effect of immunostimulatory sequences (ISS, TLR9 agonist) on dendritic cells. METHODS: Cultured mDC and pDC with/without ISS were injected intratracheally into sensitized Balb/C mice. Mice were sacrificed, and then pulmonary function tests, bronchoalveolar lavage (BAL), cell counts, and cytokine levels were evaluated. Migration of dendritic cells was also evaluated after ISS administration. RESULTS: In mice injected with mDC, airway hyperresponsiveness, eosinophil counts, and Th2 cytokine levels in BAL increased with increasing numbers of mDC injected. However, in mice injected with pDC, none of these changed, suggesting poor priming of T cells by pDC. In addition, mDC pulsed with ISS inhibited asthmatic reactions, and ISS administration inhibited migration of DC to the lung. CONCLUSIONS: We suggest that pDC played a limited role in priming T cells in this asthma model and that mDC played a major role in inducing asthma. In addition, ISS inhibited migration of DC to the lung.
