Expression of major histocompatibility complex antigen in Lewis rat cornea.
- Author:
Hungwon TCHAH
1
Author Information
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords: cornea; major histocompatability complex antigen; expression; corneal preservation media
- MeSH: Animals; Cornea/*metabolism; Culture Media; Histocompatibility Antigens Class I/*biosynthesis; Histocompatibility Antigens Class II/*biosynthesis; Immunoenzyme Techniques; Major Histocompatibility Complex; Organ Preservation/methods; Rats; Rats, Inbred Lew
- From:Korean Journal of Ophthalmology 1994;8(2):66-71
- CountryRepublic of Korea
- Language:English
- Abstract: Fresh rat corneas as well as corneas preserved in several different corneal preservation media were stained with Avidin-Biotin-peroxidase Complex method in order to evaluate major histocompatibility complex (MHC) antigen expression. In fresh corneas, class I antigen was identified in corneal epithelium, stroma and endothelium. Class II antigen was identified only in stroma. In corneas preserved in the media which contained chondroitin and dextran for 7 days, class I antigen was somewhat decreased but class II antigen was increased. In corneas preserved in the medium which contained insulin or epidermal growth factor for 7 days, class II antigens seemed to be increased compaired to the fresh cornea. Expression of MHC antigens of corneas in the medium with fetal bovine serum were similar to those of fresh corneas.
