Discrepant results by High Protein Anti-D and Low Protein Anti-D in a D Negative Newborn.
- Author:
Jin Woo YOO
1
;
Suk Woo CHOI
;
Seongsoo JANG
;
Nak Eun CHUNG
Author Information
1. Department of Clinical Pathology, Eulji University School of Medicine, Daejon, Korea.
- Publication Type:Original Article
- Keywords:
Anti-D;
High-protein reagent;
Low-protein reagent;
Anti-E;
Hemolytic disease of the newborn
- MeSH:
Antibodies, Monoclonal;
Coombs Test;
Humans;
Immune Sera;
Immunoglobulins;
Indicators and Reagents;
Infant, Newborn*;
Jaundice;
Male;
Mothers;
Phototherapy
- From:Korean Journal of Clinical Pathology
2001;21(3):221-224
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
High-protein anti-D reagents prepared from pools of human serum have been used for routine RhD typing but, low-protein, saline reactive anti-D reagents formulated predominantly with monoclonal antibodies are in current use. Because some of the high-protein reagents contain macromolecular additives that may cause red cells coated with immunoglobulin to aggregate spontaneously, antisera with these additives may produce a false-positive reaction. A four-day old male was admitted due to severe jaundice. Initially, the RhD type of the newborn using a high-protein reagent was D-positive and then, using two low-protein reagents, it was D-negative. The blood type of the mother was B, CDe, and that of the newborn was B, CcdEe. The direct antiglobulin test on the newborn's RBC was positive. Anti-E and anti-c were identified in the mother's serum and anti-E only was identified in the newborn's serum. The newborn was treated with phototherapy for 10 days and discharged as recovered. We present a case of hemolytic disease of the D negative newborn, which showed a discrepancy between high protein anti-D and low protein anti-D. With a review of literature, the newborn was possibly misinterpreted as D positive.
