Effect of Tumor Necrosis Factor-Alpha on Glomerular Epithelial Cells in Glomerular Permeability.
- Author:
Min Hyun CHO
1
;
Ji Hye LEE
;
Cheol Woo KO
;
Ja Hoon KOO
Author Information
1. Department of Pediatrics, Kyungpook National University, College of Medicine, Taegu, Korea. cwko@knu.ac.kr
- Publication Type:Original Article
- Keywords:
Glomerular epithelial cells;
TNF-alpha;
Minimal change nephrotic syndrome
- MeSH:
Child;
Epithelial Cells*;
Gene Expression;
Glomerular Basement Membrane;
Heparan Sulfate Proteoglycans;
Humans;
Nephrosis, Lipoid;
Nephrotic Syndrome;
Permeability*;
Plasma;
Recurrence;
Tumor Necrosis Factor-alpha*
- From:Journal of the Korean Society of Pediatric Nephrology
2004;8(1):1-9
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: Minimal Change Disease (MCD) is the most common primary nephrotic syndrome in children. Some suggested that tumor necrosis factor-alpha (TNF-alpha) are involved in the pathogenesis of MCD. METHODS: This study was done to see the changes of plasma and urinary TNF-alpha, and its effect on the determination of permeability of the glomerular basement membrane (BM) contributed by heparan sulfate proteoglycan (HSPG). Study patients consisted of 19 biopsy-proven MCD children aged 2-15 years old. Both plasma and urinary TNF-alpha were measured. Employing the Millicell system, TNF-alpha was screened for the permeability factors. We examined whether TNF-alpha regulated BM HSPG gene expression and HS synthesis in the glomerular epithelial cells (GECs). RESULTS: Urinary TNF-alpha during relapse was significantly increased when compared with that of during remission or controls (364.4+/-51.2 vs 155.3+/-20.8, 36.0+/-4.5 ng/mg cr) (P< 0.05). However, negative results were obtained in the permeability assay using the Millicell system. No difference was seen in the BM HSPG gene expression and HS synthesis in the GECs. CONCLUSION: It seems that TNF-alpha may not play a disease-specific role in the pathogenesis of MCD.
