Sequence variations of the bovine prion protein gene (PRNP) in native Korean Hanwoo cattle.
10.4142/jvs.2012.13.2.127
- Author:
Sangho CHOI
1
;
Hee Jong WOO
;
Jeongmin LEE
Author Information
1. Laboratory of Immunology, College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea. hjwoo@snu.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
bovine spongiform encephalopathy;
genetic polymorphisms;
prion
- MeSH:
Animals;
Base Sequence;
Cattle;
DNA/genetics;
Encephalopathy, Bovine Spongiform/*genetics;
*Genetic Variation;
Haplotypes;
Prions/*genetics;
Republic of Korea
- From:Journal of Veterinary Science
2012;13(2):127-137
- CountryRepublic of Korea
- Language:English
-
Abstract:
Bovine spongiform encephalopathy (BSE) is one of the fatal neurodegenerative diseases known as transmissible spongiform encephalopathies (TSEs) caused by infectious prion proteins. Genetic variations correlated with susceptibility or resistance to TSE in humans and sheep have not been reported for bovine strains including those from Holstein, Jersey, and Japanese Black cattle. Here, we investigated bovine prion protein gene (PRNP) variations in Hanwoo cattle [Bos (B.) taurus coreanae], a native breed in Korea. We identified mutations and polymorphisms in the coding region of PRNP, determined their frequency, and evaluated their significance. We identified four synonymous polymorphisms and two non-synonymous mutations in PRNP, but found no novel polymorphisms. The sequence and number of octapeptide repeats were completely conserved, and the haplotype frequency of the coding region was similar to that of other B. taurus strains. When we examined the 23-bp and 12-bp insertion/deletion (indel) polymorphisms in the non-coding region of PRNP, Hanwoo cattle had a lower deletion allele and 23-bp del/12-bp del haplotype frequency than healthy and BSE-affected animals of other strains. Thus, Hanwoo are seemingly less susceptible to BSE than other strains due to the 23-bp and 12-bp indel polymorphisms.
