Isolation of porcine multipotential skin-derived precursor cells and its multilineage differentiation.
- Author:
Moon Jeong CHOI
1
;
June Ho BYUN
;
Eun Ju KANG
;
Gyu Jin RHO
;
Uk Kyu KIM
;
Jong Ryoul KIM
;
Bong Wook PARK
Author Information
1. Department Oral & Maxillofacial Surgery, School of Medicine and Institute of Health Science, Gyeongsang National University, Korea. parkbw@gsnu.ac.kr
- Publication Type:In Vitro ; Original Article
- Keywords:
Skin-derived precursor cells (SKPs);
Tissue-engineering;
Adult stem cell
- MeSH:
Adipogenesis;
Adult;
Adult Stem Cells;
Bone Marrow;
Durapatite;
Ear;
Fetal Blood;
Humans;
Muscles;
Neurogenesis;
Osteocalcin;
Osteogenesis;
Osteonectin;
Peroxisome Proliferator-Activated Receptors;
Regeneration;
Regenerative Medicine;
Skin;
Stem Cells;
Swine;
Tissue Donors;
Tissue Engineering
- From:Journal of the Korean Association of Oral and Maxillofacial Surgeons
2008;34(6):588-593
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
There are increasing reports regarding regeneration of the defected tissues using tissue engineering technique. In this technique, multipotential stem cells are essential. There are many potential sources of adult stem cells, such as bone marrow, umbilical cord blood, fat, muscle, dental tissues and skin. Among them, skin is highly accessible and easily obtained with a minimum of donor site complications. Moreover, skin is an abundant adult stem cell sources and has the potential for self-replication and immune privilege. In this study, we isolated skin-derived precursor cells (SKPs) from the ear of adult miniature pigs. In these SKPs, the expression of transcriptional factors, Oct-4, Sox-2, and Nanog were detected by RT-PCR. In vitro osteogenesis and adipogenesis were observed at 3 weeks after transdifferentiations as assayed by positive von Kossa and Oil-red O staining, respectively. In addition, expression of osteocalcin and osteonectin in the osteogenic differentiation medium and PPAR GAMMA2 and aP2 in the adipogenic differentiation medium were detected by RT-PCR. In vitro neurogenesis of porcine SKPs was observed during 24 and 72 hours after treatment of neurogenic differentiation medium. The results of this study suggest that SKPs demonstrate the properties of pluripotence or multipotence and multi-lineage differentiation. This indicates that autogenous SKPs are a reliable and useful source of adult stem cells for regenerative medicine.
