Acrylamide-induced neoplastic transformation of human epithelial cells in culture.
- Author:
Sang Kyu KIM
1
;
Jin Wook KIM
;
Chin Soo KIM
Author Information
1. Department of Oral and Maxillofacial Surgery, College of Dentistry, Kyungpook National University, Korea. kimcs@knu.ac.kr
- Publication Type:Original Article
- Keywords:
Acrylamide;
carcinogenic potential;
Neoplastic transformation;
Human epithelial cell
- MeSH:
Acrylamide;
Antioxidants;
Cell Aggregation;
Epithelial Cells;
Fibronectins;
Humans;
Models, Animal;
Mouth Neoplasms;
Oxygen;
Risk Assessment;
RNA, Messenger
- From:Journal of the Korean Association of Oral and Maxillofacial Surgeons
2008;34(6):602-610
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE Acrylamide is present in significant quantities in a wide range of commonly consumed human foods. Carcinogenic risk of acrylamide through the consumption of food is a great public concern and in controversy, but it is not properly addressed due to the lack of evidence in humans. While a plenty of data is available on the carcinogenicity in animal models, the studies in humans are limited. Thus, the present study attempted to examine the carcinogenic potentials of acrylamide on the human epithelial cell, which is the target cell origin of the most cancers. MATERIAL AND METHOD & RESULT 1. Acrylamide was not cytotoxic up to 100 MICRO M as measured by MTT and LDH assays, indicating a relatively low toxicity of this substance in human epithelial cells. 2. The parameters of neoplastic cellular transformation such as cell saturation density, soft-agar colony formation and cell aggregation were analyzed to examine the carcinogenic potential of acrylamide. 3. The neoplastic transformation was further increased with the co-treatment of TPA 4. Antioxidants blocked the generation of Reactive Oxygen Species(ROS) and the GSH depleting agent dramatically increased the ROS production. 5. mRNA levels of fibronectin following acrylamide exposure was increased in a dose-dependent manner, indicating a possible biomarker of acrylamide-induced cellular transformation. CONCLUSION The present study will provide a valuable basis to compare the interspecies differences in response to carcinogenic potentials of acrylamide. The data on the interspecies differences are essential element in human risk assessment. Thus, our results obtained from the human epithelial cells will contribute to improving the risk assessment of human neoplasm including oral cancer.
