Effect of Amoebicidal Agents on the Human Corneal Keratocytes in Vitro.
- Author:
Ji Eun LEE
1
;
Jong Soo LEE
;
Hee Young CHOI
;
Hak Sun YOO
Author Information
1. Department of Ophthalmology, Pusan National University, College of Medicine, Pusan. Korea. hychoi@pusan.ac.kr
- Publication Type:In Vitro ; Original Article
- Keywords:
Acanthamoeba;
Chlorhexidine;
Human corneal keratocyte;
Minimal cysticidal concentraion;
Polyhexamethylene biguanide
- MeSH:
Acanthamoeba;
Chlorhexidine;
Corneal Keratocytes*;
Disinfectants;
Humans*;
Microscopy, Electron;
Survival Rate
- From:Journal of the Korean Ophthalmological Society
2007;48(1):125-134
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: To evaluate the effect of polyhexamethylene biguanide (PHMB) and chlorhexidine on Acanthamoeba cysts and cultured human keratocytes. METHODS: Each well of two-fold diluted PHMB and chlorhexidine were treated on the Acanthamoeba cyst suspension of 5 x 10(4) cysts/ml for 8, 24, and 48 hours to measure the minimal cysticidal concentration (MCC) of each disinfectant and was exposed to the human corneal keratocytes of 5 x 10(4) cells/ml for same hours to measure the survival rate of keratocytes. Inverted phase-contrast micrograph and electron microscopy for observing the morphologic changes were evaluated. RESULTS: MCC of PHMB was 9.42 microgram/ml, 5.62 microgram/ml, and 2.37 microgram/ml, and chlorhexidine was 24.32 microgram/ml, 10.02 microgram/ml, and 7.02 microgram/ml respecitvely in 8, 24, and 48 hours. The survival rate of keratocytes at MCC was 91.7%, 64.6%, and 49.7% in PHMB of which significant decreases were found at 24 and 48 hours, and 95.7%, 90.6%, and 78.1% in chlorhexidine of which significant decrease was only found at 48 hours. The higher the concentration of disinfectants, cysts and keratocytes demonstrated more damaged appearance. CONCLUSIONS: The amoebicidal efficacy of PHMB and chlorhexidine was similar. However, in consideration of toxic effect on keratocytes by disinfectants, chlorhexidine is suggested to be more clinically useful than PHMB.
