Effect of different sterilization methods on the surface morphology of PPDO-hybrid-PLGA nanofiber scaffold and attachments of PC12 cell.
- Author:
Juhyon LEE
1
;
Hyungi MIN
;
Juyoung JUNG
;
Nara KANG
Author Information
1. Ewha Womans University School of Medicine Department of Oral and Maxillofacial Surgery, Korea. narakang@omfs.kr
- Publication Type:Original Article
- Keywords:
Sterilization;
Nanofiber scaffold;
Surface morphology;
Attachment of cell
- MeSH:
Animals;
Ethylene Oxide;
Ethylenes;
Nanofibers;
PC12 Cells;
Sterilization
- From:Journal of the Korean Association of Oral and Maxillofacial Surgeons
2008;34(6):635-639
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVES: the effect of different sterilization methods on the surface morphology of PPDO-hybrid-PLGA nanofiber scaffold and attachments of PC12 cell were investigated. METHODS: Poly (p-dioxone)-hybrid-Poly (lactide-glycolide) (PPDO-hybrid-PLGA) nanofiber scaffold, fabricated in a tube form with 1.5 mm internal diameter, 0.2 mm thickness and 5 mm length, was prepared using electrospinning method. To study the surface morphology using SEM, The study group and control group in respective were; Control:Non-sterilized scaffold, Group I:scaffold sterilized with 70% Alcohol, Group II: scaffold sterilized with Ethylene Oxide at 65 degrees C, and Group III: scaffold sterilized with Ethylene Oxide at 37 degrees C. To investigate viability of the PC12 cell on the scaffold, The study group and control group in respective were; Control: sterilized with 70% Alcohol, Group I: sterilized with Ethylene Oxide at 65 degrees C, and Group II: sterilized with Ethylene Oxide at 37 degrees C. RESULTS: 1. The surface morphology was slightly changed in Group I, II and GroupIII, compared with control. 2. The attachment of PC12 cells in Group I, II was not higher than in control DISCUSSION: The attachment of PC12 cell is not influenced by different sterilization methods.
