Effect of Cornus Officinalis on Receptor Activator of Nuclear Factor-kappaB Ligand (RANKL)-induced Osteoclast Differentiation.
10.11005/jbm.2012.19.2.121
- Author:
Jung Young KIM
1
;
Yun Kyung KIM
;
Min Kyu CHOI
;
Jaemin OH
;
Han Bok KWAK
;
Jeong Joong KIM
Author Information
1. Department of Anatomy, School of Medicine, Wonkwang University, Iksan, Korea. jjkim@wku.ac.kr
- Publication Type:Original Article
- Keywords:
Cornus officinalis;
Osteoclast;
Osteoporosis;
RANKL
- MeSH:
Acid Phosphatase;
Blotting, Western;
Bone Resorption;
Cornus;
Cytoplasm;
Isoenzymes;
JNK Mitogen-Activated Protein Kinases;
Macrophages;
Osteoclasts;
Osteogenesis;
Osteoporosis;
Phosphorylation;
RANK Ligand;
Receptor Activator of Nuclear Factor-kappa B;
RNA, Messenger;
T-Lymphocytes
- From:Journal of Bone Metabolism
2012;19(2):121-127
- CountryRepublic of Korea
- Language:English
-
Abstract:
OBJECTIVES: Osteoporosis is a disease of bones that is thought to result from an imbalance between bone resorption and bone formation. Although osteoporosis itself has no symptoms, osteoporosis caused by osteoclasts leads to an increased risk of fracture. Here we examined the effects of cornus officinalis on receptor activator of nuclear factor-kappaB ligand (RANKL)-mediated osteoclast differentiation. METHODS: We evaluated the effects of cornus officinalis on RANKL-induced osteoclast differentiation from bone marrow-derived macrophages (BMMs) and performed a cytotoxicity assay, reverse transcriptase-polymerase chain reaction (RT-PCR), and Western blot analysis. RESULTS: Cornus officinalis significantly inhibits RANKL-mediated osteoclast differentiation in a dose-dependent manner, but without cytotoxicity against BMMs. The mRNA expression of tartrate-resistant acid phosphatase (TRAP), osteoclast-associated receptor (OSCAR), c-Fos, and nuclear factor of activated T cells cytoplasmic 1 (NFATc1) in BMMs treated with RANKL was considerably inhibited by cornus officinalis treatment. Also, cornus officinalis inhibits the protein expression of c-Fos and NFATc1. Cornus officinalis greatly inhibits RANKL-induced phosphorylation of p38 and c-JUN N-terminal kinase (JNK). Also, cornus officinalis significantly suppresses RANKL-induced degradation of I-kappaB. CONCLUSIONS: Taken together, our results suggest that cornus officinalis may be a useful the treatment of osteoporosis.
