A Study for Apoptosis and Its Mechanism of Allogeneic Activated T Lymphocytes Induced by Mouse Liver Immature Dendritic Cells.
- Author:
Young Cheol LEE
1
;
Jung Jin KIM
;
Hye Rim PARK
;
Samuel LEE
;
Young Min WOO
;
Ma Hae CHO
;
Joo Seop KIM
;
Bong Hwa LEE
Author Information
1. Department of Surgery, College of Medicine, Hallym University, Anyang, Korea. gsyclee@yahoo.co.kr
- Publication Type:In Vitro ; Original Article
- Keywords:
Liver dendritic cells;
T cell apoptosis;
Bax
- MeSH:
Animals;
Apoptosis*;
Dendritic Cells*;
DNA Fragmentation;
Liver*;
Mice*;
Spleen;
T-Lymphocytes*;
T-Lymphocytes, Cytotoxic;
Transplants
- From:Journal of the Korean Surgical Society
2004;66(1):1-4
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: Mouse liver nonparenchymal cells play an important role in the development of active apoptosis in graft- infiltrating cytotoxic T lymphocytes, and this apoptosis can be an explanation for liver graft acceptance. We intended to clarify whether immature mouse liver dendritic cells can induce apoptosis in allogeneic activated T cells and determine which mechanism is involved in this phenomenon. METHODS: A radiometric DNA fragmentation test ("JAM" assay) was used to determine whether mouse liver dendritic cells were able to induce activated T-cell apoptosis in vitro. In addition, immunohistochemical staining for Bax and Bcl-2 was examined to clarify whether Bax or Bcl-2 was involved in this apoptosis. RESULTS: Immature mouse liver dendritic cells were quite strong inducers of activated T cell apoptotic death in allogeneic mice in vitro (39.2+/-13.2% at E/T ratio=12.5/1) compared with spleen cells as effectors (4.7+/-13.4% at E/T ratio=12.5/1) (P<0.0001). By using immunohistochemical staining, we also showed that Bax might play some role in this phenonenon, but that Bcl-2 might not. CONCLUSION: Our data indicate that immature mouse liver dendritic cells might have a strong apoptotic activity toward activated T cells in allogeneic mice in vitro through a Bax-involved mechanism.
