mRNA Expression and RNA Editing (2451 C-to-U) of IL-12 Receptor beta2 in Adult Atopic Patients.
10.3346/jkms.2006.21.6.1070
- Author:
Eun Jin KIM
1
;
Won Mok LEE
;
Jung Sook HA
;
Nam Hee RYOO
;
Dong Suk JEON
;
Jae Ryong KIM
Author Information
1. Daegu Kyoungbook Blood Center, Korea Red Cross, Korea.
- Publication Type:Original Article
- Keywords:
interleukin-12 receptor;
IL-12R beta2;
Gene Expression Profiling;
Atopy;
Asthma;
Dermatitis, Atopic;
RNA Editing
- MeSH:
Sensitivity and Specificity;
Risk Factors;
Risk Assessment/*methods;
Reproducibility of Results;
Receptors, Interleukin-12/*genetics/metabolism;
RNA, Messenger/*genetics/*metabolism;
RNA Editing/genetics;
Male;
Korea/epidemiology;
Hypersensitivity, Immediate/*epidemiology/*genetics/metabolism;
Humans;
Genetic Predisposition to Disease/epidemiology/genetics;
Female;
Biological Markers/metabolism;
Adult
- From:Journal of Korean Medical Science
2006;21(6):1070-1074
- CountryRepublic of Korea
- Language:English
-
Abstract:
Interleukin (IL)-12 activates T helper (Th) 1 cells to produce interferon (IFN)-gamma which inhibits atopic inflammation. IL-12 acts through interaction with its receptor, especially beta2 subunit. In several studies, the low production of IFN-gamma in peripheral mononuclear cells of atopic patients on response to IL-12 stimulation has been reported. Therefore we investigated the IL-12 receptor beta2 (IL-12R beta2) mRNA expression and RNA editing, nucleotide 2451 C-to-U conversion, to find the cause of low responsiveness to IL-12 in atopy. Quantitative real time PCR for mRNA expression and sequence analysis for RNA editing were performed in 80 atopic patients and 54 healthy controls. The expression of IL-12R beta2 mRNA was significantly lower in atopic patients than healthy controls (p<0.05). In sequence analysis, RNA editing on nucleotide 2451 was not found from either atopic patients or healthy controls. In additional evaluation, there was no relationship between expression of IL-12R beta2 mRNA and serum total IgE or blood eosinophil count. Reduced IL-12R beta2 mRNA expression in atopic patients indicate the reduced capacity to respond to IL-12 which induce IFN-gamma production and this may contribute to Th2-skewed immune response in atopy.
