Adaptive Responses Induced by Low Dose Radiation in Dentate Gyrus of Rats.
10.3346/jkms.2006.21.6.1103
- Author:
Jin Oh KANG
1
;
Seong Eon HONG
;
Sang Ki KIM
;
Chang Ju KIM
;
Taeck Hyun LEE
;
Hyun Kyung CHANG
;
Mal Soon SHIN
;
Hong KIM
Author Information
1. Department of Radiation Oncology, Kyung Hee University Hospital, 1 Hoiki-dong, Dongdaemun-gu, Seoul, Korea. kangjino@khmc.or.kr
- Publication Type:Original Article
- Keywords:
Adaptive Response;
Radiation Effects;
Hippocampus Neurons
- MeSH:
Rats, Sprague-Dawley;
Rats;
Radiation Dosage;
Neurons/*cytology/*radiation effects;
Neuronal Plasticity/*radiation effects;
Male;
Dose-Response Relationship, Radiation;
Dentate Gyrus/*cytology/*radiation effects;
Cell Survival/radiation effects;
Cell Proliferation/*drug effects;
Animals;
Adaptation, Physiological/radiation effects
- From:Journal of Korean Medical Science
2006;21(6):1103-1107
- CountryRepublic of Korea
- Language:English
-
Abstract:
The purpose of this study is to investigate the mechanism of alternative responses to low dose irradiation for neuronal cell proliferation in the dentate gyrus of rats. To determine the effect of a single exposure to radiation, rats were irradiated with a single dose of 0.1, 1, 10 or 20 Gy. To determine the effect of the cumulative dose, the animals were irradiated daily with 0.01 Gy or 0.1 Gy from 1 to 4 days. The neuronal cell proliferation was evaluated using immunohistochemistry for 5-bromo-2'-deoxyuridine (BrdU), Ki-67 and terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) staining. Four consecutive daily irradiations with a 0.01 Gy/fraction increased the number of BrdU-positive and Ki-67-positive cells in a dose dependent manner, but this did not affect the number of TUNEL-positive cells. However, there was not a dose dependent relationship for the 0.1 Gy/fraction irradiation with the number of BrdU, Ki-67 and TUNEL positive cells. Our data support the explanation that the adaptive response, induced by low-dose radiation, in the hippocampus of rats is more likely a reflection of the perturbations of cell cycle progression.
