Transplantation of Neural Differentiated Human Mesenchymal Stem Cells into the Cochlea of an Auditory-neuropathy Guinea Pig Model.
10.3346/jkms.2011.26.4.492
- Author:
Yong Bum CHO
1
;
Hyong Ho CHO
;
Sujeong JANG
;
Han Seong JEONG
;
Jong Seong PARK
Author Information
1. Department of Otolaryngology, Chonnam National University Medical School, Gwangju, Korea. victocho@hanmail.net
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Mesenchymal Stem Cells;
Auditory neuropathy;
Hearing Loss;
Spiral ganglion neuron
- MeSH:
Animals;
Cardiotonic Agents/toxicity;
Cochlea/drug effects/pathology;
Disease Models, Animal;
Female;
Guinea Pigs;
Hearing Loss, Central/chemically induced/pathology/*therapy;
Humans;
*Mesenchymal Stem Cell Transplantation;
Mesenchymal Stem Cells/*cytology;
Neurogenesis;
Ouabain/toxicity;
Spiral Ganglion/pathology;
Transplantation, Heterologous
- From:Journal of Korean Medical Science
2011;26(4):492-498
- CountryRepublic of Korea
- Language:English
-
Abstract:
The aim of this study was to determine the effects of transplanted neural differentiated human mesenchymal stem cells (hMSCs) in a guinea pig model of auditory neuropathy. In this study, hMSCs were pretreated with a neural-induction protocol and transplanted into the scala tympani of the guinea pig cochlea 7 days after ouabain injury. A control model was made by injection of Hanks balanced salt solution alone into the scala tympani of the guinea pig cochlea 7 days after ouabain injury. We established the auditory neuropathy guinea pig model using 1 mM ouabain application to the round window niche. After application of ouabain to the round window niche, degeneration of most spiral ganglion neurons (SGNs) without the loss of hair cells within the organ of Corti and increasing the auditory brain responses (ABR) threshold were found. After transplantation of neural differentiated hMSCs, the number of SGNs was increased, and some of the SGNs expressed immunoreactivity with human nuclear antibody under confocal laser scanning microscopy. ABR results showed mild hearing recovery after transplantation. Based on an auditory neuropathy animal model, these findings suggest that it may be possible to replace degenerated SGNs by grafting stem cells into the scala tympani.
