The Effect of Placental Extract on the Expression of Tyrosinase, TRP-1 and TRP-2 in SK30 Melanoma Cells.
- Author:
Hyun Jin KIM
1
;
Jin Woo LEE
;
Young Il KIM
;
Mu Hyoung LEE
Author Information
1. Department of Dermatology, College of Medicine, Kyung Hee University, Seoul, Korea. mhlee@khmc.or.kr
- Publication Type:Original Article
- Keywords:
Placental extract;
Tyrosinase;
TRP-1;
TRP-2
- MeSH:
Cell Line;
Melanins;
Melanoma*;
Melanosis;
Monophenol Monooxygenase*;
RNA, Messenger;
Skin;
Skin Care;
Soaps
- From:Korean Journal of Dermatology
2003;41(12):1612-1618
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Placental extract is a reservoir of a large number of bioactive molecules. It has been used in skin care cosmetics and soap, it is expected to have skin whitening effect. But, the effect of placental extract on melanogenesis is not clarified yet and there are several conflicting reports. To study the effect of the placental extract on proliferation and melanogenesis of pigment cells, we examined the proliferation and change of melanin amounts and expressions of tyrosinase, tyrosinase-related protein (TRP)-1 and TRP-2 mRNA in SK30 melanoma cells, which were irradiated or not. The results were as follows: 1. The placental extract inhibited the melanogenesis of SK30 melanoma cells. 2. The placental extract showed no significant effect on the proliferation of SK30 melanoma cells. 3. The placental extract showed antimelanogenic effect by inhibiting the synthesis of tyrosinase, TRP-1 and TRP-2 mRNA. 4. The inhibitory effect of placental extract was more significant in UVB-irradiated SK30 melanoma cell lines. In conclusion, this study showed that the placental extract might be a good therapeutic regimens for UV-aggravated pigment disorders including melasma. Henceforth, further investigation is needed to identify and purify the active substance from the crude placental extract.
