Comparisons of Gut Microbiota Among Healthy Control, Patients With Conventional Adenoma, Sessile Serrated Adenoma, and Colorectal Cancer.
10.15430/JCP.2017.22.2.108
- Author:
Hyuk YOON
1
;
Nayoung KIM
;
Ji Hyun PARK
;
Yong Sung KIM
;
Jongchan LEE
;
Hyoung Woo KIM
;
Yoon Jin CHOI
;
Cheol Min SHIN
;
Young Soo PARK
;
Dong Ho LEE
;
Hyun Chae JUNG
Author Information
1. Department of Internal Medicine, Seoul National University Bundang Hospital, Seongnam, Korea. nayoungkim49@empas.com
- Publication Type:Original Article
- Keywords:
Microbiota;
Colonic neoplasms;
Colon cancer;
Colon adenoma;
Sessile serrated adenoma
- MeSH:
Adenoma*;
Bacteria;
Bacteroidetes;
Biopsy;
Carcinogenesis;
Colonic Neoplasms;
Colonoscopy;
Colorectal Neoplasms*;
Firmicutes;
Fusobacterium nucleatum;
Gastrointestinal Microbiome*;
Genes, rRNA;
Humans;
Microbiota;
Mucous Membrane;
Proteobacteria;
Pseudomonas
- From:Journal of Cancer Prevention
2017;22(2):108-114
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: Studies on gut microbiota regarding colorectal carcinogenesis, including sessile serrated adenoma (SSA), have been scarce. The aim of this study is to investigate the role of mucosa-associated gut microbiota in the colorectal carcinogenesis. METHODS: We collected biopsy samples of normal rectal mucosa during colonoscopy from healthy control and patients with conventional adenoma, SSA, and colorectal cancer (CRC), respectively (n = 6). Pyrosequencing for 16S rRNA gene of bacteria was performed to compare gut microbiota. RESULTS: The most abundant phylum in total samples was Proteobacteria (55.6%), followed by Firmicutes (27.4%) and Bacteroidetes (11.6%). There was no significant difference in relative abundance of the phylum level among the four groups. Fusobacterium nucleatum, known to be frequently detected during colorectal carcinogenesis, was found in only one sample of patient with SSA. The rarefaction curves showed that the diversity of mucosal communities of patients with CRC is the lowest among the four groups and the diversity of mucosal communities of patients with SSA is higher than that of healthy control. Among the four groups, Shannon's and Simpson's index for diversity was the lowest and the highest in the patients with CRC, respectively; it did not reach statistical significance. The proportion of genus Pseudomonas was very high in the samples of patients with stage II–IV CRC compared with those with stage I CRC (59.3% vs. 0.3%, P = 0.064). CONCLUSIONS: Our study suggests no significant role of mucosa-associated gut microbiota in the colorectal carcinogenesis. Further study for many samples or using fecal material could be helpful.
