Anthocyanins From the Fruit of Vitis coignetiae Pulliat Potentiate the Cisplatin Activity by Inhibiting PI3K/Akt Signaling Pathways in Human Gastric Cancer Cells.
10.15430/JCP.2015.20.1.50
- Author:
Jing Nan LU
1
;
Won Sup LEE
;
Arulkumar NAGAPPAN
;
Seong Hwan CHANG
;
Yung Hyun CHOI
;
Hye Jung KIM
;
Gon Sup KIM
;
Chung Ho RYU
;
Sung Chul SHIN
;
Jin Myung JUNG
;
Soon Chan HONG
Author Information
1. Department of Internal Medicine, Gyeongsang National University School of Medicine, Jinju, Korea. lwshmo@gnu.ac.kr
- Publication Type:Original Article
- Keywords:
Anthocyanins;
Cisplatin;
Stomach neoplasms
- MeSH:
Anthocyanins*;
Biological Products;
Blotting, Western;
Cell Survival;
Cisplatin*;
Fruit*;
Humans*;
Stomach Neoplasms*;
Vitis*;
X-Linked Inhibitor of Apoptosis Protein
- From:Journal of Cancer Prevention
2015;20(1):50-56
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: Cisplatin (cis-diaminedichloroplatinum, CDDP) is a widely used chemotherapeutic agent for the treatment of many cancers. However, initial resistance to CDDP is a serious problem in treating these cancers. Vitis coignetiae Pulliat (Meoru in Korea) have shown anti-nuclear factor kappa B and anti-epidermal growth factor receptor activities in cancer cells. METHODS: In this study, in order to seeking an approach to increase the anti-cancer effects of CDDP with natural products. Here, we investigated anthocyanins isolated from Vitis coignetiae Pulliat (anthocyanidins isolated from meoru, AIMs) can enhance anti-cancer effects of cisplatin (CDDP) in stomach cancer cells. The cell viability of SNU-1 and SNU-16 cells after treated with AIMs and CDDP were analyzed by MTT assay. The expressions of Akt and X-linked inhibitor of apoptosis protein (XIAP) proteins were examined by western blot in AIMs- and CDDP-treated cells. RESULTS: We found that AIMs enhanced anticancer effects of CDDP, which activity was additive but not synergistic. AIMs suppressed Akt activity of the cancer cells activated by CDDP. AIMs also suppressed in XIAP an anti-apoptotic protein. CONCLUSIONS: This study suggests that the anthocyanins isolated from fruits of Vitis coignetiae Pulliat enhanced anti-cancer effects of CDDP by inhibiting Akt activity activated by CDDP.
