Effects of Scytosiphon lomentaria on osteoblastic proliferation and differentiation of MC3T3-E1 cells.
10.4162/nrp.2016.10.2.148
- Author:
Mi Hwa PARK
1
;
Seoyeon KIM
;
Jihyeon CHEON
;
Juyeong LEE
;
Bo Kyung KIM
;
Sang Hyeon LEE
;
Changsuk KONG
;
Yuck Yong KIM
;
Mihyang KIM
Author Information
1. Department of Food and Nutrition, College of Medical and Life Science, Silla University, 140 Baegyang-daero, 700beon-gil, Sasang-Gu, Busan 46958, Korea. mihkim@silla.ac.kr
- Publication Type:Original Article
- Keywords:
Scytosiphon lomentaria;
MC3T3-E1 cell;
differentiation;
alkaline phosphatase;
mineralization
- MeSH:
Alkaline Phosphatase;
Bone and Bones;
Bone Morphogenetic Protein 2;
Bone Remodeling;
Bone Resorption;
Cell Proliferation;
Collagen Type I;
Osteoblasts*;
Osteogenesis;
Osteoprotegerin;
Transcription Factors
- From:Nutrition Research and Practice
2016;10(2):148-153
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND/OBJECTIVES: Bone formation and bone resorption continuously occur in bone tissue to prevent the accumulation of old bone, this being called bone remodeling. Osteoblasts especially play a crucial role in bone formation through the differentiation and proliferation. Therefore, in this study, we investigated the effects of Scytosiphon lomentaria extract (SLE) on osteoblastic proliferation and differentiation in MC3T3-E1 cells. MATERIALS/METHODS: A cell proliferation assay, alkaline phosphatase (ALP) activity assay, alizarin red staining and protein expression analysis of osteoblastic genes were carried out to assess the osteoblastic proliferation and differentiation. RESULTS: The results indicated that treatment of SLE promoted the proliferation of MC3T3-E1 cells and improved ALP activity. And, SLE treatment significantly promoted mineralized nodule formation compared with control. In addition, cells treated with SLE significantly upregulated protein expression of ALP, type 1 collagen, bone morphogenetic protein 2, runt-related transcription factor 2, osterix, and osteoprotegerin. CONCLUSIONS: The results demonstrate that SLE promote differentiation inducement and proliferation of osteoblasts and, therefore may help to elucidate the transcriptional mechanism of bone formation and possibly lead to the development of bone-forming drugs.
