A Study on the Expression of Proliferating Cell Nuclear Antigen and Apoptosis of the Hepatocellular Carcinoma in Human and Hepatitis B Virus X Transgenic Mice.
- Author:
Hyung Bae MOON
1
;
Dae Yeul YU
;
Hyung Ryun YOO
;
Byung Joon SO
;
Kwon Mook CHAE
;
Haak Cheol KIM
;
Ki Jung YUN
;
Won Cheol HAN
;
Hyang Jeong JO
;
Bo Yong KIM
Author Information
1. Departments of Pathology, Surgery, and Internal Medicine, Wonkwang University, School of Medicine, Iksan 570-749, Korea. hbmoon@wonkwang.ac.kr
- Publication Type:Original Article
- Keywords:
Transgenic;
Hepatocellular;
Carcinoma;
Proliferating cell nuclear antigen;
Apoptosis
- MeSH:
Animals;
Apoptosis*;
Carcinoma, Hepatocellular*;
Formaldehyde;
Hepatitis B virus*;
Hepatitis B*;
Hepatitis*;
Humans*;
Kinetics;
Liver;
Mice;
Mice, Transgenic*;
Proliferating Cell Nuclear Antigen*
- From:Korean Journal of Pathology
2001;35(2):129-136
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: This experiment was designed to study the cell kinetics of hepatocellular carcinoma (HCC) in both hepatitis B virus X (HBx) transgenic mice and humans. METHODS: The immunohistochemical stain of proliferating cell nuclear antigen (PCNA) and TdT-mediated dUTP-biotin nick end labeling (TUNEL) assay of apoptosis were used on formalin fixed-paraffin embedded tissues. RESULTS: PCNA labeling indices (PCNA-LI) in the liver of HBx transgenic mice were markedly increased in HCC (11.3%) compare to the dysplastic areas (1.3%) and in the liver of non-transgenic littermates (0.1%). There was no significant difference of PCNA-LI in the dysplastic areas between HCC developed mice and non-HCC developed mice. Apoptosis labeling indices (Apoptosis-LI) in both the dysplastic areas and HCC of HBx transgenic mice were similar to those of non-transgenic littermates. PCNA-LI was markedly increased in human HCC (28.9%) compare to the background of HCC (2.9%) and the control liver (2.9%). Apoptosis-LI was decreased in human HCC (0.3%) compare to the background of HCC (0.4%) and the control liver (1.0%). Conclusion : There is a marked increase of cell proliferating activity in human HCC and in HCC of HBx transgenic mice, and there is a decrease of apoptosis in human HCC, but not in HCC of HBx transgenic mice.
