Expression of Neutrophil Gelatinase-Associated Lipocalin in Calcium-Induced Keratinocyte Differentiation.
10.3346/jkms.2008.23.2.302
- Author:
Jeung Hoon LEE
1
;
Kyung Chae KYE
;
Eun Young SEO
;
Kyungmoon LEE
;
Sang Keun LEE
;
Jong Soon LIM
;
Young Joon SEO
;
Chang Deok KIM
;
Jang Kyu PARK
Author Information
1. Department of Dermatology, School of Medicine, Chungnam National University, Daejeon, Korea. parkjk@cnu.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
LCN2 Protein, Human;
Keratinocytes
- MeSH:
Acute-Phase Proteins/*biosynthesis;
Calcium/*metabolism;
Cell Differentiation;
Culture Media;
Culture Media, Conditioned;
Enzyme-Linked Immunosorbent Assay;
*Gene Expression Regulation;
Homeostasis;
Humans;
Keratinocytes/enzymology;
Lipocalins/*biosynthesis;
Models, Biological;
Proto-Oncogene Proteins/*biosynthesis;
Psoriasis/enzymology;
Reverse Transcriptase Polymerase Chain Reaction;
Skin/*metabolism;
Skin Neoplasms/enzymology
- From:Journal of Korean Medical Science
2008;23(2):302-306
- CountryRepublic of Korea
- Language:English
-
Abstract:
In a previous search for the differentially expressed genes in keratinocyte differentiation, we identified neutrophil gelatinase-associated lipocalin (NGAL) as a calcium- induced gene. In this study, we further verified the expression of NGAL in cultured keratinocytes as well as in several skin diseases. Reverse transcription-polymerase chain reaction (RT-PCR), Western blot, and ELISA clearly showed that NGAL expression was markedly increased in calcium-induced keratinocyte differentiation in vitro. However, in our previous report, NGAL expression was not detected in normal skin tissue except for hair follicle by in situ hybridization and immunohistochemistry, indicating the difference of cell status between in vitro and in vitro conditions. Interestingly, NGAL expression was highly increased in psoriasis-like inflammatory disorders (lichen planus and pityriasis rubura pilaris) and skin cancers (keratoacanthoma and squamous cell carcinoma), implying that NGAL may be related with the epidermal hyperplasia. Collectively, these results reveal the potential importance of NGAL in the maintenance of skin homeostasis.
